DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-FLAG® M2 Antibody): FITC
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Catalog number
SPC-720D-FITC
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Price
Please ask
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Size
100 µg
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Alernative name
Rabbit Anti- DYKDDDDK Tag Polyclonal
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Target
DYKDDDDK Tag
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Conjugate
FITC
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Research Area s
Tags and Cell Markers , Epitope Tags
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Category
Antibodies
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Product Type
Polyclonal
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Immunogen
Synthetic peptide corresponding to the DYKDDDDK Tag, conjugated to Keyhole Limpet Haemocyanin (KLH).
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Immunogen Species
NA
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Gene ID
NA
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Swiss Prot
NA
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Applications
WB
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Host Species
Rabbit
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Species Reactivity Full Name
Species Independent
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Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
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Concentration
1 mg/ml
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Specificity
Binds to DYKDDDDK (FLAG tag) conjugated proteins. Recongizes the same epitope as Sigma's Anti-FLAG® antibodies.
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Storage Temperature
-20°C
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Shipping Temperature
Blue Ice or 4°C
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Anti-FITC Antibody, Flag
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French translation
anticorps
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Gene target
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Short name
DYKDDDDK Tag Antibody (Equivalent Sigma's Anti- ® M2 Antibody): FITC
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Technique
Antibody, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, Fluorescein, Tags
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Label
tag
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Alternative name
DYKDDDDK detection labelled (antibody to-) (Equivalent to Sigma's Antibody toFLAG® M2 (antibody to-)): fluorecein
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Alternative technique
antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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