DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-FLAG® M2 Antibody): ATTO 655

• Catalog number
  SPC-720D-A655
• Price
  Please ask
• Size
  100 µg

• Alernative name
  Rabbit Anti- DYKDDDDK Tag Polyclonal
• Target
  DYKDDDDK Tag
• Conjugate
  ATTO 655
• Research Area s
  Tags and Cell Markers , Epitope Tags
• Category
  Antibodies
• Product Type
  Polyclonal
• Immunogen
  Synthetic peptide corresponding to the DYKDDDDK Tag, conjugated to Keyhole Limpet Haemocyanin (KLH).
• Immunogen Species
  NA
• Gene ID
  NA
• Swiss Prot
  NA
• Applications
  WB
• Host Species
  Rabbit
• Species Reactivity Full Name
  Species Independent
• Storage Buffer
  PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration
  1 mg/ml
• Specificity
  Binds to DYKDDDDK (FLAG tag) conjugated proteins. Recongizes the same epitope as Sigma's Anti-FLAG® antibodies.
• Storage Temperature
  -20°C
• Shipping Temperature
  Blue Ice or 4°C
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag
• French translation
  anticorps

• Gene target
  DYKDDDDK   Tag   Equivalent   Sigma's   ATTO   655  
• Short name
  DYKDDDDK Tag Antibody (Equivalent Sigma's Anti- ® M2 Antibody): ATTO 655
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Tags
• Label
  tag
• Alternative name
  DYKDDDDK detection labelled (antibody to-) (Equivalent to Sigma's Antibody toFLAG® M2 (antibody to-)): ATTO 655
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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