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Target
AKT1 (pThr308)
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Conjugate
PerCP
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Research Area s
Cell Signaling, Protein Phosphorylation, Serine / Threonine Kinases, PKB / AKT, Cancer, Apoptosis, Cancer, Cancer Metabolism, Cancer, Cell Cycle, PI3k/AKT Pathway
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Alternative Name s
RAC-PK-alpha Antibody, Protein kinase B alpha Antibody, RAC Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, RAC Alpha Antibody, AKT Antibody, EC:2.7.11.1 Antibody, AKT 1 Antibody, PRKBA Antibody, AKT1_HUMAN Antibody, AKT1 Antibody, PKB Antibody, PKB-ALPHA Antibody, MGC99656 Antibody, PKB alpha Antibody, RAC-alpha serine/threonine-protein kinase Antibody
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Category
Antibodies
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Product Type
Polyclonal
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Immunogen
Synthetic phospho-peptide from human AKT1 around the phosphorylation site of threonine 308 (pThr308).
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Immunogen Species
Human
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Accession Number
NP_001014431.1
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Gene ID
207
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Swiss Prot
P31749
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Applications
WB, IHC, ICC/IF
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Host Species
Rabbit
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Species Reactivity
Human, Mouse, Rat
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Antibody Dilution
WB (1:250-1:500); IHC (1:25-1:50); ICC/IF (1:50-100); optimal dilutions for assays should be determined by the user.
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Purification
Peptide Affinity Purified
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Storage Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
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Specificity
Detects endogenous levels of AKT1 only when phosphorylated at threonine 308 (pThr308).
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Storage Temperature
-20ºC
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Shipping Temperature
Blue Ice or 4ºC
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Certificate of Analysis
A 1:500 dilution of SPC-1341 was sufficient for detection of AKT1 (pThr308) in 10 µg of human embryonic kidney epithelial cell line (hek293) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
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Cellular Localization
Nucleus, Plasma Membrane, Cell Membrane, Cytoplasm, Cytoskeleton, Ciliary Basal Body, Microtubule, Spindle, Cytosol, Mitochondrion, Nucleoplasm, Cell-Cell Junction, Postsynapse, Protein Complex, Vesicle
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Field of Use
For in vitro research use only.
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Gross Weight kg
1.4
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Net Weight g
0.1
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Properties
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional conjugate
Peridinin chlorophyll (PerCP)
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French translation
anticorps
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Gene target
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Gene symbol
AKT1
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Short name
AKT1 Antibody (pThr308): PerCP
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Technique
Antibody, Percp, antibodies against human proteins, antibodies for, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the AKT1 Antibody (pThr308): PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMarq.
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Isotype
IgG
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Label
PerCP
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Alternative name
v-akt murine thymoma viral oncogene homolog 1 (antibody to-) (pThr308): PerCP
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Alternative technique
antibodies, antibodies-percp
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Alternative to gene target
v-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, phosphatidylinositol-3, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and
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