AKT1 Antibody (pThr308): Biotin

• Catalog number
  SPC-1341D-BI
• Price
  Please ask
• Size
  100 µl

• Target
  AKT1 (pThr308)
• Conjugate
  Biotin
• Research Area s
  Cell Signaling, Protein Phosphorylation, Serine / Threonine Kinases, PKB / AKT, Cancer, Apoptosis, Cancer, Cancer Metabolism, Cancer, Cell Cycle, PI3k/AKT Pathway
• Alternative Name s
  RAC-PK-alpha Antibody, Protein kinase B alpha Antibody, RAC Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, RAC Alpha Antibody, AKT Antibody, EC:2.7.11.1 Antibody, AKT 1 Antibody, PRKBA Antibody, AKT1_HUMAN Antibody, AKT1 Antibody, PKB Antibody, PKB-ALPHA Antibody, MGC99656 Antibody, PKB alpha Antibody, RAC-alpha serine/threonine-protein kinase Antibody
• Category
  Antibodies
• Product Type
  Polyclonal
• Immunogen
  Synthetic phospho-peptide from human AKT1 around the phosphorylation site of threonine 308 (pThr308).
• Immunogen Species
  Human
• Accession Number
  NP_001014431.1
• Gene ID
  207
• Swiss Prot
  P31749
• Applications
  WB, IHC, ICC/IF
• Host Species
  Rabbit
• Species Reactivity
  Human, Mouse, Rat
• Antibody Dilution
  WB (1:250-1:500); IHC (1:25-1:50); ICC/IF (1:50-100); optimal dilutions for assays should be determined by the user.
• Purification
  Peptide Affinity Purified
• Storage Buffer
  PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
• Specificity
  Detects endogenous levels of AKT1 only when phosphorylated at threonine 308 (pThr308).
• Storage Temperature
  -20ºC
• Shipping Temperature
  Blue Ice or 4ºC
• Certificate of Analysis
  A 1:500 dilution of SPC-1341 was sufficient for detection of AKT1 (pThr308) in 10 µg of human embryonic kidney epithelial cell line (hek293) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
• Cellular Localization
  Nucleus, Plasma Membrane, Cell Membrane, Cytoplasm, Cytoskeleton, Ciliary Basal Body, Microtubule, Spindle, Cytosol, Mitochondrion, Nucleoplasm, Cell-Cell Junction, Postsynapse, Protein Complex, Vesicle
• Field of Use
  For in vitro research use only.
• Technical data
• SDS
• Gross Weight kg
  1.4
• Net Weight g
  0.1
• Properties
  If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  AKT1   pThr308  
• Gene symbol
  AKT1
• Short name
  AKT1 Antibody (pThr308):
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  IgG
• Label
  Biotin
• Alternative name
  v-akt murine thymoma viral oncogene homolog 1 (antibody to-) (pThr308): biotinilated
• Alternative technique
  antibodies
• Alternative to gene target
  v-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, phosphatidylinositol-3, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and

Gene info

• Identity
  HGNC:391
• Gene
  AKT1
• Long gene name
  AKT serine/threonine kinase 1
• Synonyms gene name
  • v-akt murine thymoma viral oncogene homolog 1
• Synonyms
  • RAC
  • PKB
  • PRKBA
  • AKT
  • RAC-alpha
• Synonyms name
  • protein kinase B
• GenBank acession
  • M63167
• Locus
  14q32.33
• Discovery year
  1986-01-01
• Entrez gene record
  207
• Pubmed identfication
  • 21214427
• RefSeq identity
  • NM_005163
• Classification
  • Pleckstrin homology domain containing
  • AKT kinases
• VEGA ID
  OTTHUMG00000170795
• Locus Specific Databases
  • LRG_721

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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