Mouse Recombinant AHA1 Protein
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Catalog numberSPR-311C
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PricePlease ask
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Size2x100 µg
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Stock availabilityIn Stock
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Scientific contextAha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
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Protein targetAHA1
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Protein reactivityMouse
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Certificate of analysisThis product has been certified >90% pure using SDS-PAGE analysis. 2uM SPR-311 generated a 9-fold ATPase activation of 2uM HSP90 (His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)
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Protein descriptionMouse Recombinant AHA1 Protein
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Other nameAha1 Protein, SHSA1 Protein, HSPC322 Protein, p38 Protein
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Primary research areaCancer, Heat Shock, Cell Signaling, Trafficking, Chaperones
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CategoryProtein
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Brand namenone
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OriginRecombinant
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NCBI numberNP_666148.1
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Gene number217737
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Protein numberQ8BK64
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Verified applicationsWB, SDS-PAGE, Functional Assay
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Relevant bio activityAHA1 Protein
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Protein expression modelE. coli
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Protein charastericsSee included datasheet.
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Peptide sequenceMGHHHHHHMAKWGEGDPRWIVEERADATNVNNWHWTERDASNWSTEKLKTLFLAVRVENEEGKCEVTEVNKLDGEASINNRKGKLIFFYEWTIKLNWTGTSKSGVQYKGHVEIPNLSDENSVDEVEISVSLAKDEPDTNLVALMKEDGVKLLREAVGIYISTLKTEFTQGMILPTVNGESVDPVGQPALKTETCKAKSAPSKSQAKPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFTHAPAALEADRGGKFHMVDGNVTGEFTDLVPEKHIAMKWRFKSWPEGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF
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Protein purificationAffinity Purified
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Purity pourcentage>90% High purity
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Recommended buffer for storage25mM Hepes buffer pH7.2, 100mM KoAc, 50% glycerol
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Protein concentrationLot/batch specific. See included datasheet.
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Protein specificity~38 kDa
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Protein tagHis tag
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Supplementary useful informationPlease see included datasheet or contact us
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Protein cell localizationCytoplasm
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Bibliography1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
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Release date1-Aug-2011
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PubMed numberNot added. Please refer to PubMed
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Tested applicationsto be tested
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Tested species reactivityto be tested
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Representative figure legendSDS-PAGE of ~38kDa his-tagged mouse Aha1 protein (SPR-311). SDS-Page of mouse AHA1 Protein (SPR-311)
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WarningsNon-hazardous materials
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Protein originCanada
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Total weight kg1.4
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Net weight g0.2
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolAHSA1, AHSA2P
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Short nameMouse Recombinant AHA1 Protein
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TechniqueRecombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. StressMark proteins advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Hostmouse
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SpeciesMouse, Mouses
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Alternative nameMouse Rec. AHA1 Protein
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Alternative techniquerec, murine
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Gene info
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Identity
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Gene
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Long gene nameactivator of HSP90 ATPase activity 1
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Synonyms gene
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Synonyms gene name
- chromosome 14 open reading frame 3
- AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-07-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameactivator of HSP90 ATPase homolog 2, pseudogene
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Synonyms gene
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Synonyms gene name
- AHA1, activator of heat shock 90kDa protein ATPase homolog 2 (yeast)
- activator of HSP90 ATPase homolog 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-04-04
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Entrez gene record
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Pubmed identfication
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data