Rat Anti-Drosophila HSP70 Monoclonal IgG2B, Clone 7FB
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Catalog numberSMC-230D-RPE
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PricePlease ask
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Size100 µg
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Clone7FB
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ImmunogenPrepared from Drosophila tissue culture cells heat shocked at 36.5◦C for 3 hours, and isolated using SDS PAGE.
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Antibody s full descriptionRat Anti-Drosophila HSP70 Monoclonal IgG2B Antibody, Clone: 7FB: RPE
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameHSP70Bb Antibody, Heat Shock Protein 70Bb Antibody, dHSP70 Antibody, HSP70b Antibody, HSP70B Antibody, Dm-HSP70 Antibody
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Raised inRat
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Antibody s targetHSP70
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Primary research fieldsCancer, Heat Shock
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BrandnameNone
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Antibodies applicationsWB, ICC/IF, ELISA
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Antibody s reactivityFruit Fly (Drosophila melanogaster)
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Antibody s dilutionsWB (1:2000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~70kDa (heat-inducible form).
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-230 was sufficient for detection of Drosophila HSP70 using an indirect assay with rabbit anti-rat IgG and goat anti-rabbit IgG:HRP.
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Antibody in cellSee included datasheet or contact our support service
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextHSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at more.
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Bibliography1. Welch W.J. and Suhan J.P. (1986) J Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J.Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell 59: 591-601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Galan A., et al. (2000) J. Biol. Chem. 275: 11418-11424. 8. Kondo T., et al. (2000) J. Biol. Chem. 275: 8872-8879. 9. Misaki T., et al. (1994) Clin. Exp. Immun. 98: 234-239. 10. Pockley A.G., et al. (1998) Immunol. Invest. 27: 367-377. 11. Moon I.S., et al. (2001) Cereb Cortex 11(3): 238-248. 12. Dressel et al. (2000) J. Immunol. 164: 2362-2371. 13. Verma A.K., et al. (2007) Fish and Shellfish Immunology. 22(5): 547-555. 14. Banduseela V.C., et al. (2009) Physiol Genomics. 39(3): 141-159.
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Released date22-Oct-2014
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PubMed number26234525
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Tested applicationsWestern Blot
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Tested reactivityDrosophila
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NCBI numberNP_524927.2
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Gene number48582
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Protein numberQ9BIS2
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Antibody s datasheetContact our support service
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Gene target
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Gene symbolHSPA1B, HSPA1A, HSPA14, HSPA1L
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Short nameAnti- HSP70 Monoclonal IgG2B, Clone 7FB
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Techniqueanti-, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies
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HostRat
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IsotypeIgG2B, IgG2b
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LabelRPE
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SpeciesDrosophila, Drosophila subspecies d. Megalonaster or the fruit fly is used in genetic research because of the short life cycle and the possibility of knock-out and knock-ins with Cas9 for ends-in gene targets like CRISPR.Cas9 and TALEN vectors . Drosophilas, Rats
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Alternative nameRat Antibody toDrosophila HSP70 monoclonal IgG2B, clonality 7FB
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Alternative techniqueantibodies
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Clone name7FB
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Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 1B
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Synonyms gene name
- heat shock 70kD protein 1B
- heat shock 70kDa protein 1B
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Synonyms
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Locus
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Discovery year1995-08-11
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Entrez gene record
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Pubmed identfication
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Classification
- TIM23 complex
- Heat shock 70kDa proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 1A
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Synonyms gene
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Synonyms gene name
- heat shock 70kD protein 1A
- heat shock 70kDa protein 1A
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Classification
- TIM23 complex
- Heat shock 70kDa proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 14
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Synonyms gene name
- heat shock 70kDa protein 14
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-04-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Heat shock 70kDa proteins
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 1 like
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Synonyms gene name
- heat shock 70kD protein-like 1
- heat shock 70kDa protein 1-like
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-05-12
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Entrez gene record
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Pubmed identfication
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Classification
- TIM23 complex
- Heat shock 70kDa proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data