Rabbit Anti-Yeast STI1P (HOP) Polyclonal: ATTO 390
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Catalog numberSPC-203D-A390
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Price:
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Size100 µl
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ImmunogenYeast Sti1p raised against peptide(CDINQSNSMPKEPET)-KLH fusion
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Antibody s targetYeast HOP
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Antibody s full descriptionRabbit Anti-Yeast STI1P (HOP) Polyclonal
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Primary research fieldsCancer, Heat Shock
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameSTIP1 Antibody, Sti1 yeast Antibody, Hop Antibody, HSC70/HSP90 organizing protein Antibody, Epididymis secretory sperm binding protein Li 94n antibody, HEL S 94n antibody, Hop antibody, HSC70/HSP90 organizing protein antibody, HSC70/HSP90-organizing protein antibody, IEF SSP 3521 antibody, NY REN 11 antigen antibody, P60 antibody, Renal carcinoma antigen NY-REN-11 antibody, STI1 antibody, STI1L antibody, STIP1 antibody, Stress induced phosphoprotein 1 antibody, Stress-induced-phosphoprotein 1 antibody, Transformation sensitive protein IEF SSP 3521 antibody, Transformation-sensitive protein IEF SSP 3521 antibody
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Verified applicationsWB, ELISA, FCM
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Raised inRabbit
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Antibody s reactivityMouse, Yeast
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Antibody s recommended dilutions for useWB (1:2500); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromRabbit Antiserum
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Recommended buffer for storageRabbit Antiserum, PBS, 50% glycerol
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects ~ 66kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysis0.5 µg/ml of SPC-203 was sufficient for detection of Sti1p (HOP) in 20 µg of W303 yeast lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm, Nucleus
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextThe stress inducible protein Sti1p is also commonly known as HSP70-HSP90 organization protein (HOP). It is located in diverse cellular regions and can move between the cytoplasm and the nucleus. It functions to reversibly link together the protein chaperones HSP70 and HSP90. HOP contains three tetratricopeptide repeat (TPR) domains, TPR1, TPR2a and TPR2b. HSP70 binding has been localized to TRP1 and sp90 binding have been localized to TPR2a (1). It has also been found to modulate the chaperone activities of the linked proteins and possible interacts with other chaperones and proteins. It has also been found to participate in other complexes besides the HSP70/HSP90 one (2). HOP is closely related to human 63kDa protein that is sensitive to simian virus SV40 transformation, and is related to the yeast heat-shock- responsive STI1 gene product (3, 4).
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Bibliography1. Flom G., Behal R.H., Rosen L., Cole D.G., Johnson J.L. (2007) Biochem J. 404(1): 159-167. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt3): 789-796. 3. Honore B.H., et al. (1992) J Biol Chem. 267: 8485- 8491. 4. Nicolet C.M., et al. (1989) Mol Cell Bio. 9: 3638-3646.
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Released date2/Apr/2013
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NCBI numberNP_014670.1
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Gene number854192
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Protein numberP15705
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PubMed number27665558|27665558
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Tested applicationsELISA|Flow Cytometry
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Tested reactivityMouse|Mouse
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure linkNo Data Available
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolSTIP1, HOPX
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Short nameRabbit Anti-Yeast STI1P (HOP) Polyclonal: ATTO 390
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TechniquePolyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits, Ascomycota
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IsotypeImmunoglobulin
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LabelATTO 390
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SpeciesYeast, Yeasts
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Alternative nameproduction species: rabbit Antibody toYeast STI1P (HOP) polyclonal: ATTO 390
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene namestress induced phosphoprotein 1
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Synonyms gene name
- stress-induced-phosphoprotein 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-08-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tetratricopeptide repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHOP homeobox
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Synonyms
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Synonyms name
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Locus
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Discovery year2007-08-20
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Entrez gene record
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Pubmed identfication
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Classification
- PRD class homeoboxes and pseudogenes
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data