Rabbit Anti-Yeast AHA1p Polyclonal: FITC

  • Catalog number
    SPC-204D-FITC
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    Yeast Aha1 raised against peptide (CESQVKSNYTRGNQK)-KLH fusion
  • Antibody s target
    Yeast AHA1p
  • Antibody s full description
    Rabbit Anti-Yeast AHA1p Polyclonal
  • Primary research fields
    Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Activator of 90 kDa heat shock protein ATPase homolog 1 Antibody, Aha1 Antibody, SHSA1 Antibody, HSPC322 Antibody, p38 Antibody
  • Verified applications
    WB
  • Raised in
    Rabbit
  • Antibody s reactivity
    Yeast
  • Antibody s recommended dilutions for use
    WB (1:2500); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Rabbit Antiserum
  • Recommended buffer for storage
    Rabbit Antiserum, PBS, 50% glycerol
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects ~ 38kDa. Can run up to 45kDa on SDS Page.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    0.5 µg/ml of SPC-204 was sufficient for detection of Aha1p in 20 µg of W303 yeast lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm, Endoplasmic Reticulum
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
  • Bibliography
    1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
  • Released date
    2/Apr/2013
  • NCBI number
    NP_010500.3
  • Gene number
    851800
  • Protein number
    Q12449
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    No Data Available
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    Yeast   AHA1p  
  • Short name
    Rabbit Anti-Yeast AHA1p Polyclonal: FITC
  • Technique
    Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits, Ascomycota
  • Isotype
    Immunoglobulin
  • Label
    FITC
  • Species
    Yeast, Yeasts
  • Alternative name
    production species: rabbit Antibody toYeast AHA1p polyclonal: fluorecein
  • Alternative technique
    polyclonals, rabbit-anti, antibodies, fluorescine
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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