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Immunogen
Synthetic peptide conjugated to KLH. The peptide target is specific to the HSC70 of salmonid fish.
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Antibody s target
Salmon HSC70
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Antibody s full description
Rabbit Anti-Salmon HSC70 (Salmon) Polyclonal
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Primary research fields
Cancer, Heat Shock
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody, HSPA8 Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Fish, Salmon, Three-spined stickleback (Gasterosteus aculeatus)
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Rabbit Antiserum
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Recommended buffer for storage
Lyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~73kDa. The antibody detects HSC70, but not HSP70.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
Contact our support service for certificate of analysis
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Antibody in cell
Cytoplasm, Melanosome
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (5).
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Bibliography
1. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 2. Rothman J. (1989) Cell 59: 591-601. 3. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 4. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA 89: 7290-7294. 5. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.
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Released date
1/Jan/2009
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NCBI number
NP_001133079.1
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Gene number
100194521
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Protein number
B5DFX7
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PubMed number
26123780
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Tested applications
Western Blot
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Tested reactivity
Gasterosteus aculeatus (Three-spined stickleback)
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Salmon tissue lysates showing detection of HSC70 protein using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-303). Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-303) at 1:1000. Rabbit Anti-HSC70 Antibody used in Western blot (WB) on Salmon tissue lysates (SPC-303)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Conjugation
not conjugated