Rabbit Anti-Rat PDI Polyclonal: FITC
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Catalog numberSPC-114D-FITC
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Price:
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Size100 µl
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ImmunogenRat PDI synthetic peptide conjugated to KLH
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Antibody s targetRat PDI
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Antibody s full descriptionRabbit Anti-Rat PDI Polyclonal
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Primary research fieldsCell Signaling, Trafficking, Chaperones, Organelle Markers
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Antibody s categoryPolyclonal Antibodies
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Antibody s other namePDA2 Antibody, PDI Antibody, PDIA2 Antibody, PDIP Antibody, pancreatic protein disulfide isomerase Antibody
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Verified applicationsWB, IHC, ICC/IF, IP
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Raised inRabbit
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Antibody s reactivityHuman, Mouse, Rat, Bovine, Dog, Hamster, Monkey, Guinea Pig (Cavia porcellus), Sheep, Pig, African clawed frog (Xenopus laevis), Mollusk, Mussel (Perna viridis)
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Antibody s recommended dilutions for useWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~58kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-114 was sufficient for detection of PDI in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
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Antibody in cellEndoplasmic Reticulum, Endoplasmic Reticulum Lumen
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextThe three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5). PDI is an abundant 55kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).
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Bibliography1. NA K.S. et al. (2007) Mol Cells. 24(2): 261-7. 2. Mayer M., Kies U., Kammermeier R., and Buchner J. (2000) J Biol Chem. 275(38): 29421-5. 3. Delom F., Mallet B., Carayon P., and Lejeune P.J. (2001) J Biol Chem 276(24): 21337-42. 4. Schultz-Norton J.R., McDonald W.H., Yates J.R. and Nardulli A.M. (2006) Mol Endocrinol 20(9): 1982-95. 5. Turano C., Coppari S. Altieri F. and Ferraro (2002) J Cell Physiol 193: 154-163. 6. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819. 7. Yoshimori T., et al. (1990) J Biol Chem. 265(26): 15984-90.
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Released date1/Jun/2008
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NCBI numberNP_001099245.2
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Gene number287164
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Protein numberP04785
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PubMed number25678563|25678563|21684241
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Tested applicationsWestern Blot|Western Blot|Western Blot
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Tested reactivityHuman|Mouse|Perna viridis (Asian green mussel)
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PDI Polyclonal Antibody (SPC-114). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (SPC-114) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-PDI Antibody. (C) Composite. | Western blot analysis of Rat tissue mix showing detection of PDI protein using Rabbit Anti-PDI Polyclonal Antibody (SPC-114). Load: 15 µg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (SPC-114) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PDI Polyclonal Antibody (SPC-114). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (SPC-114) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-PDI Antibody. (C) Composite. Rabbit Anti-PDI Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-114) | Rabbit Anti-PDI Antibody used in Western blot (WB) on Rat tissue mix (SPC-114) | Rabbit Anti-PDI Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-114)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesThis StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameOryctolagus cuniculus, Rattus norvegicus
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Gene target
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Gene symbolPDIA2, PADI1, ERP29, P4HB
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Short nameRabbit Anti- PDI Polyclonal: FITC
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TechniquePolyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelFITC
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SpeciesRat, Rats
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Alternative nameproduction species: rabbit Antibody toRat PDI polyclonal: fluorecein
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Alternative techniquepolyclonals, rabbit-anti, antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameprotein disulfide isomerase family A member 2
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Synonyms gene
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Synonyms gene name
- protein disulfide isomerase, pancreatic
- protein disulfide isomerase-associated 2
- protein disulfide isomerase family A, member 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-12-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein disulfide isomerases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepeptidyl arginine deiminase 1
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Synonyms gene name
- peptidyl arginine deiminase, type I
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-03-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Peptidyl arginine deiminases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendoplasmic reticulum protein 29
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Synonyms gene
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Synonyms gene name
- chromosome 12 open reading frame 8
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-04-27
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Entrez gene record
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Pubmed identfication
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Classification
- Protein disulfide isomerases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprolyl 4-hydroxylase subunit beta
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Synonyms gene
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Synonyms gene name
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase-associated 1)
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide
- prolyl 4-hydroxylase, beta polypeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein disulfide isomerases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data