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Immunogen
Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).
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Antibody s target
Rat NKCC2
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Antibody s full description
Rabbit Anti-Rat NKCC2 Polyclonal
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Primary research fields
Cancer, Neuroscience, Pumps/Transporters, Ion Pumps/Transporters
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
SLC12A1 Antibody, sodium potassium chloride cotransporter 2 Antibody, sodium-potassium-chloride cotransporter isoform 2 Antibody, BSC1 Antibody, MBSC1 Antibody, Na-K-2Cl cotransporter Antibody, NKCC2A variant A Antibody, RBSC Antibody
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Verified applications
WB, Immunohistochemistry
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~160kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
1 µg/ml of SPC-401 was sufficient for detection of NKCC2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).
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Bibliography
1. Caceres P.S., Ares G.R., and Ortiz P.A. (2009) J Biol Chem. 284(37): 24965-24971. 2. Xue H., et al. (2009) Neurogastroentrol Motil. 21(10): 1068-1089.
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Released date
11/Jun/2010
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NCBI number
NP_062007
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Gene number
25065
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Protein number
Q5PQV1
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PubMed number
26984496|26183401|26207612|26207612|24808538|24808538
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Tested applications
Immunohistochemistry|Western Blot |Immunohistochemistry|Western Blot|Immunohistochemistry|Western Blot
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Tested reactivity
Rat|Mouse|Mouse|Mouse|Mouse|Mouse
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2 protein using Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401). Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401) at 1:1000. Rabbit Anti-NKCC2 Antibody used in Western blot (WB) on Rat kidney tissue lysates (SPC-401)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Oryctolagus cuniculus, Rattus norvegicus
