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Immunogen
Produced against a synthetic peptide mapping to the N-temrinal of the alpha subunit (amino acids 46-68) of rat Alpha ENaC (antibody designation 3560-2).
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Antibody s target
Rat ENaC
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Antibody s full description
Rabbit Anti-Rat ENaC alpha Polyclonal
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Primary research fields
Neuroscience, Ion Channels, Sodium Channels, Epithelial Sodium Channels (ENaC)
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
SCNN1A Antibody, Epithelial Sodium Channel-α Antibody, Epithelial Sodium Channel alpha Antibody, Alpha ENaC 2 Antibody, Alpha ENaC Antibody, Alpha NaCH Antibody, Alpha-ENaC Antibody, Amiloride sensitive epithelial sodium channel alpha subunit Antibody, Amiloride sensitive sodium channel subunit alpha Antibody, Amiloride-sensitive sodium channel subunit alpha Antibody, ENaCa Antibody, ENaCalpha Antibody, Epithelial Na(+) channel subunit alpha Antibody, Epithelial Na+ channel subunit alpha Antibody, FLJ21883 Antibody, Nonvoltage gated sodium channel 1 subunit alpha Antibody, Nonvoltage-gated sodium channel 1 subunit alpha Antibody, SCNEA Antibody, SCNN 1 Antibody, SCNN1 Antibody, SCNN1A Antibody, SCNNA_HUMAN Antibody, Sodium channel nonvoltage gated 1 alpha Antibody
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Verified applications
WB, IHC, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Rat, Mouse, African clawed frog (Xenopus laevis)
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Antibody s recommended dilutions for use
WB (1:1000), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~85kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
1 µg/ml of SPC-403 was sufficient for detection of alpha-ENaC in 35 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Apical Cell Membrane
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
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Bibliography
1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
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Released date
11/Jun/2010
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NCBI number
NP_113736
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Gene number
25122
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Protein number
Q6IRJ1
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PubMed number
27895120|27443990|27440776|27635187|27053687|26963391|26755736|26418861|26207612|26207612|25941340|24707975|25164814|25122048|24808538|24958889|24060890|24060890|24145067|23558339|23264685|23171554|26069797|22814399|23029554|22549242
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Tested applications
Western Blot|Western Blot|Western Blot|Western Blot|Western Blot|Western Blot|Western Blot|Western Blot|Immunohistochemistry|Western Blot|Western Blot|Immunohistochemistry|Western Blot|Western Blot|Western Blot|Gel Shift|Immunohistochemistry|Western Blot|Immunohistochemistry|Immunohistochemistry|Western Blot|Western Blot |Western Blot|Western Blot|Western Blot|Western Blot
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Tested reactivity
Mouse|Rat|Rat|Human|Mouse|Mouse|Mouse|Mouse|Mouse|Mouse|Mouse|Rat|Mouse|Mouse|Mouse|Mouse|Mouse|Mouse|Rat|Mouse|Mouse|Mouse|Mouse|Xenopus laevis|Mouse|Rat
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-403). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-403) at 1:1000. | Western blot analysis of Mouse kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-403). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-403) at 1:1000. Rabbit Anti-ENaC Antibody used in Western blot (WB) on Rat kidney tissue lysates (SPC-403) | Rabbit Anti-ENaC Antibody used in Western blot (WB) on Mouse kidney tissue lysates (SPC-403)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
The Rabbit Anti- ENaC alpha Polyclonal: ATTO 390 is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Oryctolagus cuniculus, Rattus norvegicus
