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Immunogen
Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2
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Antibody s target
Rat Aquaporin 2
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Antibody s full description
Rabbit Anti-Rat Aquaporin 2 Polyclonal
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Primary research fields
Neuroscience, Ion Channels, Aquaporins
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ADH water channel Antibody, AQP 2 Antibody, AQP CD Antibody, AQP-2 Antibody, AQP-CD Antibody, AQP2 Antibody, AQP2_HUMAN Antibody, AQPCD Antibody, Aquaporin 2 collecting duct Antibody, Aquaporin CD Antibody, Aquaporin-2 Antibody, Aquaporin-CD Antibody, Aquaporin2 Antibody, Aquaporine 2 Antibody, Collecting duct water channel protein Antibody, MGC34501 Antibody, Water channel aquaporin 2 Antibody, Water channel protein for renal collecting duct Antibody, WCH CD Antibody, WCH-CD Antibody, WCHCD Antibody
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Verified applications
WB, IHC, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~28.8kDa. May detect larger glycosylated bands ~35-50kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
0.5 µg/ml of SPC-503 was sufficient for detection of aquaporin 2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Membrane, Apical Cell Membrane, Basolateral Cell Membrane, Cytoplasmic Vesicle, Cytoplasmic Vesicle Membrane, Golgi Apparatus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell (3). Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI) (4).
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Bibliography
1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396. 2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258. 3. Lodish H.F. (2008) Molecular Cell Biology. New York: W.H. Freeman. Print. 445. 4. www.vivo.colostate.edu/hbooks/molecules/aquaporins.html
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Released date
6/Dec/2011
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NCBI number
NP_037041.2
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Gene number
25386
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Protein number
P34080
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PubMed number
27413198|27277500|25006961|22549242
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Tested applications
|Immunocytochemistry/Immunofluorescence|Western Blot|Western Blot
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Tested reactivity
|Mouse|Mouse|Rat
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green). | Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 2 protein using Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503). Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503) at 1:2000. Showing glycosylated and non-glycosylated bands. Rabbit Anti-Aquaporin 2 Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-503) | Rabbit Anti-Aquaporin 2 Antibody used in Western blot (WB) on Rat kidney inner medullary homogenates (SPC-503)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Oryctolagus cuniculus, Rattus norvegicus
