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Immunogen
Recombinant Hsp21 protein derived from the sequence of Arabidopsis thaliana
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Antibody s target
Plant HSP21
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Antibody s full description
Rabbit Anti-Plant HSP21 (Plant) Polyclonal
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Primary research fields
Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
17.6kDa class II heat shock protein Antibody, HSP17.6II Antibody, Heat Shock Protein 21 Antibody, Chloroplast localized small heat shock protein antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Plant, Arabidopsis thaliana
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Antibody s recommended dilutions for use
WB (1:3000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Rabbit Antiserum
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Recommended buffer for storage
Lyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~21kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
Contact our support service for certificate of analysis
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Antibody in cell
Cytoplasmic
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
HSP21 is a chloroplast-localized small heat shock protein. How these small HSPs protect living cells is not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small HSPs are also produced late in the seed maturation process. HSP21 has also been shown to protect Photosystem II from temperature-dependent oxidative stress as well as having a role in fruit reddening and the conversion of chloroplasts to chromoplasts (1).
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Bibliography
1. Neta-Sharir T., Isaacson S., Lurie D., Weiss. (2005) Plant Cell 17: 1829-38.
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Released date
1/Jan/2009
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NCBI number
NP_194497.1
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Gene number
828881
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Protein number
P31170
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Plants RNA, DNA , cDNA and plant tissues are used to study plant genes. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Group
Polyclonals and antibodies
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Latin name
Oryctolagus cuniculus
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Conjugation
not conjugated
