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Immunogen
Recombinant protein Arabidopsis thaliana HSP101/ClpB C-terminal
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Antibody s target
Plant HSP101
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Antibody s full description
Rabbit Anti-Plant HSP101/ClpB CT (Plant) Polyclonal
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Primary research fields
Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
heat shock protein 1010 Antibody, HOT1 Antibody, HSP101 Antibody, HSPS Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Plant, Arabidopsis thaliana, Monocot (Agave tequilana)
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Rabbit Antiserum
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Recommended buffer for storage
Lyophilized rabbit Antiserum. For reconstitution add 200 µl of sterile water.
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects the C-terminal domain of HSP101 ~101kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
1 µl/ml of SPC-305 was sufficient for detection of HSP101 in 2 µg of Arabidopsis thaliana WT lysate stressed at 38°C for 1.5 hours by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
HSPs/chaperones are known to be expressed in plants not only when they experience high temperatures stress, but also in response to a wide range of other environmental stresses such as water stress, salt stress, cold stress and oxidative stress (1, 2). HSP100 is one such major class that acts as a chaperone. HSP101/ClpB is a member of this HSP100 family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones. In spite of expression during heat stress, members of HSP100 protein family are also expressed during seed development. HSP101 protein is localized in a cytoplasm.
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Bibliography
1. Vierling E. (1991) Annu Rev. Plant Physiol. Plant Mol. Biol. 42: 579-620. 2. Waters W., et al. (2004) Trends Plant Sci. 9: 244-252.
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Released date
1/Jan/2009
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NCBI number
NP_565083.1
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Gene number
843771
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Protein number
P42730
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Arabidopsis Arabidopsis thalina tissue showing detection of HSP101 protein using Rabbit Anti-HSP101 Polyclonal Antibody (SPC-305). Load: 2 µg. Primary Antibody: Rabbit Anti-HSP101 Polyclonal Antibody (SPC-305) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP-conjugated Goat Anti-Rabbit at 1:10000. From left to right: Arabidopsis thaliana WT stressed at 38°C for 1.5 hour (1); Hsp101 null mutant. Band showing at ~101kDa. Rabbit Anti-HSP101 Antibody used in Western blot (WB) on Arabidopsis Arabidopsis thalina tissue (SPC-305)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.2
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Plants RNA, DNA , cDNA and plant tissues are used to study plant genes. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Group
Polyclonals and antibodies
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Latin name
Oryctolagus cuniculus
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Conjugation
not conjugated
