Rabbit Anti-P. Falciparum HSP40 Polyclonal: ATTO 594
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Catalog numberSPC-184D-A594
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Price:
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Size100 µg
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ImmunogenC-terminal peptide of Pf11_0513 conjugated to KLH
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Antibody s targetP. falciparum HSP40
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Antibody s full descriptionRabbit Anti-P. Falciparum HSP40 Polyclonal
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Primary research fieldsCancer, Heat Shock
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameDNAJ1 Antibody, NDAJB1 Antibody, HDJ1 Antibody, HSP40 Antibody, HSPF1 Antibody, DnaJ homolog subfamily B member 1 Antibody, Dna J protein homolog 1 Antibody, Heat shock 40 kDa protein 1 Antibody, HSP40 Antibody, heat shock protein 40 Antibody, Human DnaJ protein 1 Antibody, hDj-1 Antibody
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Verified applicationsWB, ICC/IF
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Raised inRabbit
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Antibody s reactivityPlasmodium falciparum
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Antibody s recommended dilutions for useWB (1:2000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromProtein A Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1.8 mg/ml
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Antibody s specificityDetects ~ 62kDa. Specific to P. Falciparum and does not cross-react to any protein from Human erythrocytes.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysis0.9 µg/ml of SPC-184 was sufficient for detection of Pf11_0513 in 20 µg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm, Nucleus
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextDnaJ/HSP40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, HSP70s. Because the ATP hydrolysis is essential for the activity of HSP70s, DnaJ/HSP40 proteins actually determine the activity of HSP70s by stabilizing their interaction with substrate proteins. DnaJ/HSP40 proteins all contain the J domain through which they bind to HSP70s. HSP40, also known as HDJ1 (1), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-6). HSP 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (3). PF11_0513 belongs to the HSP40 family of chaperones. This protein has a Plasmodium export element (PEXEL). It is exported out of the parasite into the infected erythrocytic compartment.
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Bibliography1. Ohtsuka K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240. 2. Melville M.W., et al. (1997) PNAS USA. 94: 97-102. 3. Hattori H., Let al. (1992) Cell Structure and Function. 17: 77-86. 4. Ohtsuka K. Masuda A., Nakai A., and Nagata K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647. 5. Bardwell J.C.A., et al. (1986) J. Biol. Chem. 261: 1782-1785. 6. Ohku M., Tamura F., Nishimura S., and Uchida H. (1986) J. Biol. Chem. 261: 1778-1781.
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Released date27/Apr/2009
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NCBI numberXP_001348169.3
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Gene number811046
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Protein numberQ8IHM7
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure legendWestern blot analysis of Parasite lysates showing detection of HSP40 protein using Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184). Primary Antibody: Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184) at 1:2000. Rabbit Anti-HSP40 Antibody used in Western blot (WB) on Parasite lysates (SPC-184)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolDNAJB1
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Short nameRabbit Anti-P. Falciparum HSP40 Polyclonal: ATTO 594
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TechniquePolyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 594
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Alternative nameproduction species: rabbit Antibody toP. Falciparum HSP40 polyclonal: ATTO 594
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameDnaJ heat shock protein family (Hsp40) member B1
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Synonyms gene
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Synonyms gene name
- DnaJ (Hsp40) homolog, subfamily B, member 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-10-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DNAJ (HSP40) heat shock proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data