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Immunogen
Synthetic peptide from the N-terminal of Human WDFY3 (aa. 533-544)
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Antibody s target
Human WDFY3
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Antibody s full description
Rabbit Anti-Human WDFY3 Polyclonal
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Primary research fields
Cancer, Autophagy, Cardiovascular System, Heart
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
WDFY3 Antibody, Alfy Antibody, WD repeat and FYVE domain-containing protein 3 Antibody, Autophagy-linked FYVE protein Antibody, KIAA0993 Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Human, Rat
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~400 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-666 was sufficient for detection of WDFY3 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus Membrane, Cytoplasm, Nucleus, Pml Body, Membrane, Peripheral Membrane Protein, Cytoplasmic Side
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Tissue specificity
Ubiquitous. Expressed in osteoclast and their mononuclear precursors (at protein level).
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Scientific context
See included datasheet or contact our support service.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_055806.2
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Gene number
23001
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Protein number
Q8IZQ1
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 395.2 kDa WDFY3 protein using Rabbit Anti-WDFY3 Polyclonal Antibody (SPC-666). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody (SPC-666) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 395.2 kDa. Rabbit Anti-WDFY3 Antibody used in Western blot (WB) on HeLa and 293Trap cell lysates (SPC-666)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
WDFY3-AS1, WDFY3, WDFY3-AS2
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Short name
Rabbit Anti- WDFY3 Polyclonal: ATTO 680
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
ATTO 680
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman WDFY3 polyclonal: ATTO 680
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Alternative technique
polyclonals, rabbit-anti, antibodies
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