-
Immunogen
Synthetic peptide from the C-terminal of Human VMP1 (aa. 391-402)
-
Antibody s target
Human VMP1
-
Antibody s full description
Rabbit Anti-Human VMP1 Polyclonal
-
Primary research fields
Cancer, Tumor Biomarkers, Cardiovascular System, Heart, Cancer, Autophagy, Cell Signaling, Metabolism, Metabolism processes
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
TDC1 Antibody, Transmembrane protein 49 Antibody, TMEM49 Antibody, VMP1 Antibody, HSPC292 Antibody, Vacuole membrane protein 1 Antibody, ORF Names: Antibody
-
Verified applications
WB
-
Raised in
Rabbit
-
Antibody s reactivity
Human
-
Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration
1 mg/ml
-
Antibody s specificity
Detects ~46 kDa.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:1000 dilution of SPC-680 was sufficient for detection of VMP1 in 15 µg of Human 293T Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane, Multi-Pass Membrane Protein, Cell Membrane, Multi-Pass Membrane Protein, Vacuole Membrane, Multi-Pass Membrane Protein, Endoplasmic Reticulum
-
Scientific context
See included datasheet or contact our support service.
-
Bibliography
Refer to PubMed
-
Released date
29/Aug/2016
-
NCBI number
NP_112200.2
-
Gene number
81671
-
Protein number
Q96GC9
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
-
Representative figure legend
Western blot analysis of Rat Pancreas cell lysates showing detection of 46.2kDa VMP1 protein using Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 46.2kDa. Other Band(s): Multiple bands for A549; Rat Brain shows a band at 60kDa, 75kDa, and 150kDa. Rabbit Anti-VMP1 Antibody used in Western blot (WB) on Pancreas cell lysates (SPC-680)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Conjugation
Biotinylated
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
