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Immunogen
Synthesized peptide derived from human TGF β3.
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Antibody s target
Human TGF beta 3
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Antibody s full description
Rabbit Anti-Human TGF beta 3 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ARVD Antibody, ARVD1 Antibody, FLJ16571 Antibody, MGC105479 Antibody, MGC118722 Antibody, prepro-transforming growth factor beta-3 Antibody, RNHF Antibody, TGF beta 3 Antibody, TGF beta3 Antibody, TGF-beta-3 Antibody, TGFB 3 Antibody, Tgfb3 Antibody, TGFB3_HUMAN Antibody, transforming growth factor beta 3 Antibody, Transforming growth factor beta-3 Antibody
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Verified applications
WB, IHC, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~50kDa, endogenous levels of total TGF β3 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1313 was sufficient for detection of TGF beta 3 in 10 µg of Cos7 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Secreted
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Transforming growth factor beta-3 is a protein that in humans is encoded by the TGFB3 gene. It is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. TGFBR3 is extensively modified by glycosaminoglycan groups (GAG). TGF-β3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-β3, mammals develop a deformity known as a cleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse.
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Bibliography
1. Bandyopadhyay B et al. (2006) The Journal of Cell Biology 172 (7): 1093–105. 2. Herpin A et al. (2004) Developmental and Comparative Immunology 28 (5): 461–85. 3. Taya Y et al. (1999) Development 126 (17): 3869–79. 4. Dudas M et al. (2004) Developmental Biology 266 (1): 96–108. 5. Kaartinen V et al. (1995) Nature Genetics 11 (4): 415–21.
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Released date
6/Sep/2016
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NCBI number
NP_003230.1
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Gene number
7043
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Protein number
P10600
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-TGF beta 3 Polyclonal Antibody (SPC-1313). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-TGF beta 3 Polyclonal Antibody (SPC-1313) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Monkey Cos7 cell lysates showing detection of ~12.5kDa TGF beta 3 protein using Rabbit Anti-TGF beta 3 Polyclonal Antibody (SPC-1313). Lane 1: Monkey Cos7 cells. Lane 2: Monkey Cos7 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-TGF beta 3 Polyclonal Antibody (SPC-1313) at 1:1000. Predicted/Observed Size: ~12.5kDa . Rabbit Anti-TGF beta 3 Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1313) | Rabbit Anti-TGF beta 3 Antibody used in Western blot (WB) on Cos7 cell lysates (SPC-1313)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
