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Immunogen
Synthesized peptide derived from human TGF β1 antibody.
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Antibody s target
Human TGF beta 1
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Antibody s full description
Rabbit Anti-Human TGF beta 1 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
CED Antibody, DPD1 Antibody, LAP Antibody, Latency-associated peptide Antibody, Prepro transforming growth factor beta 1 Antibody, TGF beta 1 Antibody, TGF beta Antibody, TGF beta 1 protein Antibody, TGF-beta 1 protein Antibody, TGF-beta-1 Antibody, TGF-beta-5 Antibody, TGF-beta1 Antibody, TGFB Antibody, Tgfb-1 Antibody, tgfb1 Antibody, TGFB1_HUMAN Antibody, TGFbeta Antibody, TGFbeta1 Antibody, Transforming Growth Factor b1 Antibody, Transforming Growth Factor beta 1 Antibody, Transforming growth factor beta 1a Antibody, transforming growth factor beta-1 Antibody, transforming growth factor, beta 1 Antibody
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Verified applications
WB, IHC, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~45kDa, endogenous levels of total TGF β1 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1312 was sufficient for detection of TGF beta 1 in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Secreted
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Tissue specificity
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage.
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Scientific context
Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
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Bibliography
1. Ghadami M. et al. (2000) Am. J. Hum. Genet. 66 (1): 143–7. 2. Vaughn SP. et al. (2000) Genomics 66 (1): 119–21. 3. Assoian RK. et al. (1983) J. Biol. Chem. 258 (11): 7155–60. 4. Wahl SM et al. (1988) J. Immunol. 140 (9): 3026–32. 5. Tiemessen MM et al. (2003) Int. Immunol. 15 (12): 1495–504. 6. McGonigle S et al. (2001) FEBS Lett. 490 (1-2): 65–9.
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Released date
6/Sep/2016
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NCBI number
NP_000651.3
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Gene number
7040
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Protein number
P01137
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human HepG2 cell lysates showing detection of ~44kDa TGF beta 1 protein using Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312). Lane 1: Human HepG2 cells. Lane 2: Human HepG2 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312) at 1:1000. Predicted/Observed Size: ~44kDa. Rabbit Anti-TGF beta 1 Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1312) | Rabbit Anti-TGF beta 1 Antibody used in Western blot (WB) on HepG2 cell lysates (SPC-1312)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
