Rabbit Anti-Human SOD1 (UbetaB) Polyclonal: PE/ATTO 594
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Catalog numberSPC-205D-P594
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Price:
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Size100 µg
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ImmunogenN-terminal region of SOD1, unfolded beta barrel region
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Antibody s targetHuman SOD1
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Antibody s full descriptionRabbit Anti-Human SOD1 (UbetaB) Polyclonal
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Primary research fieldsCancer, Oxidative Stress, Neuroscience, Neurodegeneration, ALS Disease
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameUSOD Antibody, Superoxide dismutase 1 Antibody, SOD Antibody, SOD1 unfolded beta barrel region Antibody
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Verified applicationsWB, ICC/IF, ELISA
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Raised inRabbit
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s recommended dilutions for useWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromProtein A Purified
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Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityRecognizes a conformation specific epitope where the beta barrel is unfolded.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysis1µg/ml of SPC-205 was sufficient for detection of the unfolded beta barrel of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextSuperoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as Anti-SOD1 (UbetaB), which targets an unfolded region of the beta barrel of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with Anti-SOD1 (EDI) (SPC-206D) which detects an exposed dimer interface (EDI) of SOD1.
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Bibliography1. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 2. Furukawa Y., and O’Halloran T. (2006) Antioxid Redox Signal. 8(5-6):847-67. 3. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol. 284:L917-L925. 4. Hassan H.M. (1988) Free Radical Biol. Med. 5:377-385. 5. Kerman A., et al. (2010) Acta Neuropathol. 119:335-344.
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Released date25/Sep/2013
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NCBI numberCAG46542
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Gene number6647
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Protein numberP00441
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PubMed number28057013
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Tested applicationsELISA
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Tested reactivityHuman
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (SPC-205). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (SPC-205) at 1:110 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Mitochondrion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD1 (UBB) Antibody. (C) Composite. | Western blot analysis of Human, Rat, Mouse Hela cells, Brain, Lung showing detection of ~18 kDa SOD1 (UBB) protein using Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (SPC-205). Lane 1: Molecular Weight Ladder. Lane 2: Human HeLa Cell Lysates. Lane 3: Rat Brain. Lane 4: Mouse Lung. Load: 20 µg. Primary Antibody: Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (SPC-205) at 1:1000. Predicted/Observed Size: ~18 kDa. Rabbit Anti-SOD1 (UBB) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-205) | Rabbit Anti-SOD1 (UBB) Antibody used in Western blot (WB) on Human, Rat, Mouse Hela cells, Brain, Lung (SPC-205)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolSOD1-DT, SOD1
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Short nameRabbit Anti- SOD1 (UbetaB) Polyclonal: PE/ATTO 594
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelPE/ATTO 594
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman superoxide dismutase 1, soluble (UbetaB) polyclonal: peroxidase/ATTO 594
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Alternative to gene targetsuperoxide dismutase 1, soluble, ALS and ALS1 and HEL-S-44 and homodimer and hSod1 and IPOA and SOD, SOD1 and IDBG-1609 and ENSG00000142168 and 6647, chaperone binding, nuclei, Sod1 and IDBG-174662 and ENSMUSG00000022982 and 20655
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Gene info
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Identity
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Gene
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Long gene nameSOD1 divergent transcript
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Locus
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Discovery year2021-07-01
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Entrez gene record
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Classification
- Divergent transcripts
Gene info
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Identity
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Gene
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Long gene namesuperoxide dismutase 1
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Synonyms gene
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Synonyms gene name
- amyotrophic lateral sclerosis 1 (adult)
- superoxide dismutase 1, soluble
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Superoxide dismutases
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data