Rabbit Anti-Human SOD (Mn) Polyclonal: ATTO 680
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Catalog numberSPC-118D-A680
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Price:
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Size100 µg
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ImmunogenHuman Mn SOD
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Antibody s targetHuman SOD2
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Antibody s full descriptionRabbit Anti-Human SOD (Mn) Polyclonal
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Primary research fieldsCancer, Oxidative Stress, Cell Signaling, Neuroscience, Neurodegeneration, Cardiovascular System
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameManganese SOD Antibody, IPO B Antibody, Mn SOD Antibody, SOD2 Antibody
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Verified applicationsWB, IHC, IP, ELISA
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Raised inRabbit
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Antibody s reactivityHuman, Rat, Mouse, Bovine, Dog, Chicken, Gerbil, Guinea Pig (Cavia porcellus), Pig, Hamster, Monkey, Rabbit, Sheep, African clawed frog (Xenopus laevis), Frog, Wood Frog (Rana sylvatica)
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Antibody s recommended dilutions for useWB (1:5000), IHC (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromProtein A Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~25kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysis0.2 µg/ml of SPC-118 was sufficient for detection of Mn SOD in 20 µg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-mouse IgG:AP as the secondary antibody.
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Antibody in cellMitochondrion, Mitochondrion Matrix
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextSuperoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).
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Bibliography1. Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. Furukawa Y., O’Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Biomedical Research. 11: 187-194 7. Wispe J.R., et al. (1989) BBA. 994: 30-36. 8. Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.
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Released date1/Jun/2007
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NCBI numberNP_000627.2
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Gene number6648
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Protein numberP04179
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PubMed number25316288
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Tested applicationsWestern Blot
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Tested reactivityNorth American wood frog (Rana sylvatica)
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure legendImmunohistochemistry analysis using Rabbit Anti-SOD2 Polyclonal Antibody (SPC-118). Tissue: muscle fibres. Species: Rat. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody (SPC-118) at 1:100. Left: Untreated, Right: treated with 3mmol*kg-1 NAC. Courtesy of: E. Barreiro, IMIM, Spain.. Rabbit Anti-SOD2 Antibody used in Immunohistochemistry (IHC) on Rat muscle fibres (SPC-118)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolSOD3
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Short nameRabbit Anti- SOD (Mn) Polyclonal: ATTO 680
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 680
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman antioxidant enzyme (Mn) polyclonal: ATTO 680
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene namesuperoxide dismutase 3
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Synonyms gene name
- superoxide dismutase 3, extracellular
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Synonyms
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Locus
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Discovery year1988-05-08
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Entrez gene record
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Classification
- Superoxide dismutases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data