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Immunogen
A synthesized peptide derived from RUNX2
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Antibody s target
Human RUNX2
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Antibody s full description
Rabbit Anti-Human RUNX2 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Acute myeloid leukemia 3 protein Antibody, Alpha subunit 1 Antibody, AML3 Antibody, CBF alpha 1 Antibody, CBF-alpha-1 Antibody, CBFA1 Antibody, CCD Antibody, CCD1 Antibody, Cleidocranial dysplasia 1 Antibody, Core binding factor Antibody, Core binding factor runt domain alpha subunit 1 Antibody, Core binding factor subunit alpha 1 Antibody, Core-binding factor subunit alpha-1 Antibody, MGC120022 Antibody, MGC120023 Antibody, Oncogene AML 3 Antibody, Oncogene AML-3 Antibody, OSF 2 Antibody, OSF-2 Antibody, OSF2 Antibody, Osteoblast specific transcription factor 2 Antibody, Osteoblast-specific transcription factor 2 Antibody, OTTHUMP00000016533 Antibody, PEA2 alpha A Antibody, PEA2-alpha A Antibody, PEA2aA Antibody, PEBP2 alpha A Antibody, PEBP2-alpha A Antibody, PEBP2A1 Antibody, PEBP2A2 Antibody, PEBP2aA Antibody, PEBP2aA1 Antibody, Polyomavirus enhancer binding protein 2 alpha A subunit Antibody, Polyomavirus enhancer-binding protein 2 alpha A subunit Antibody, Runt domain Antibody, Runt related transcription factor 2 Antibody, Runt-related transcription factor 2 Antibody, RUNX2 Antibody, RUNX2_HUMAN Antibody, SL3 3 enhancer factor 1 alpha A subunit Antibody, SL3-3 enhancer factor 1 alpha A subunit Antibody, SL3/AKV core binding factor alpha A subunit Antibody, SL3/AKV core-binding factor alpha A subunit Antibody
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Verified applications
WB, IHC, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:200), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~56kDa, endogenous levels of total RUNX2
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1311 was sufficient for detection of RUNX2 in 10 µg of MC3T3-E1 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus
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Tissue specificity
Specifically expressed in osteoblasts.
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Scientific context
RUNX2 (Runt-related transcription Factor) is a transcription factor that binds to its canonical consensus sequence 5’- PYGPYGGT-3’ in a number of promoters and enhancers. Through interaction with Twist 1 and Twist 2, RUNX2 regulates osteocalcin transcription, following parathyroid hormone signaling for bone loss repair. Ossification in skeletal formation is also partially regulated through RUNX2. Essentially, this transcription factor is responsible for many developmental and repair mechanisms in bone and collagen. Mutations in Cbfa1/Runx2 are associated with the disease Cleidocranial dysostosis.
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Bibliography
1. Green RE et al. (2010) Science 328 (5979): 710–22. 2. Pockwinse SM et al. (2011) Journal of Cellular Physiology 226 (5): 1383–9. 3. Lian JB. (2004) Critical Reviews in Eukaryotic Gene Expression 14 (1-2): 1–41.
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Released date
6/Sep/2016
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NCBI number
NP_001019801.3
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Gene number
860
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Protein number
Q13950
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus