Rabbit Anti-Human RAS Polyclonal: FITC

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• Catalog number
  SPC-173D-FITC
• Price:

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• Size
  100 µg

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• Immunogen
  Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)
• Antibody s target
  Human RAS
• Antibody s full description
  Rabbit Anti-Human RAS Polyclonal
• Primary research fields
  Cancer, Cell Signaling, Epigenetics
• Antibody s category
  Polyclonal Antibodies
• Antibody s other name
  GTPase Hras Antibody, GTPase Kras Antibody, GTPase Nras Antibody, Ha Ras Antibody, K Ras Antibody, K ras p21 Antibody, KRAS Antibody, NRAS Antibody, p21ras Antibody, RASH2 Antibody
• Verified applications
  WB, IHC, ICC/IF
• Raised in
  Rabbit
• Antibody s reactivity
  Human, Mouse, Rat, Bovine
• Antibody s recommended dilutions for use
  WB (1:1000), IHC (1:100), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
• Antibody s purified from
  Protein G Purified
• Recommended buffer for storage
  PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~21kDa.
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Certificate of analysis
  1 µg/ml of SPC-173 was sufficient for detection of Ras in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Antibody in cell
  Cell Membrane, Golgi Apparatus, Golgi Apparatus Membrane
• Tissue specificity
  See included datasheet or contact our support service.
• Scientific context
  The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
• Bibliography
  1. Boguski M.S. and McCormick F. (1993) Nature 366: 643–654. 2. Avruch J., et al. (1994) Trends Biochem. Sci.19: 279–283. 3. Buday L. and Downward J. (1993) Cell 73: 611–620. 4. Huang D.C., et al. (1993) Mol. Cell Biol. 13: 2420–2431. 5. Bos J.L. (1989) Cancer Res. 49: 4682–4689. 6. Ma J. and Karplus M. (1997) J. Mol. Biol. 274:114–131.
• Released date
  26/Jul/2012
• NCBI number
  NP_035067.2
• Gene number
  18176
• Protein number
  B2KGV5
• PubMed number
  Refer to PubMed
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• Antibody s datasheet
  Contact our support service to receive datasheet or other technical documentation.
• Representative figure link
  more | details | see here | read more | look at
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:200 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Lipid-anchor. Focal adhesion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-RAS Antibody. (C) Composite. | Immunohistochemistry analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Basal cell of the epidermis. | Western blot analysis of Mouse Brain Cortex cell lysates showing detection of ~21 kDa RAS protein using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Cortex cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cell-cell junction. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Lipid-anchor. Focal adhesion. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-RAS Antibody. (C) Composite. Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-173) | Rabbit Anti-RAS Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-173) | Rabbit Anti-RAS Antibody used in Western blot (WB) on Brain Cortex cell lysates (SPC-173) | Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SPC-173) | Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-173)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availabilit
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  StressMark antibodies supplies antibodies that are for research of human proteins.
• Properties
  This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Anti-FITC Antibody
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

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• Gene target
  RAS  
• Gene symbol
  NRAS, RRAS, RASGRF2, MRAS
• Short name
  Rabbit Anti- RAS Polyclonal: FITC
• Technique
  anti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  Immunoglobulin
• Label
  FITC
• Species
  Human, Humans
• Alternative name
  production species: rabbit Antibody toHuman RAS polyclonal: fluorecein
• Alternative technique
  polyclonals, rabbit-anti, antibodies, fluorescine

Gene info

• Identity
  HGNC:7989
• Gene
  NRAS
• Long gene name
  NRAS proto-oncogene, GTPase
• Synonyms gene name
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • neuroblastoma RAS viral oncogene homolog
• Synonyms
  • N-ras
• GenBank acession
  • BC005219
• Locus
  1p13.2
• Discovery year
  2001-06-22
• Entrez gene record
  4893
• Pubmed identfication
  • 6705568
  • 11524732
• RefSeq identity
  • NM_002524
• Classification
  • RAS type GTPase family
• VEGA ID
  OTTHUMG00000012059
• Locus Specific Databases
  • NRASbase: Mutation registry for autoimmune lymphoproliferative syndrome type IV
  • LRG_92

Gene info

• Identity
  HGNC:10447
• Gene
  RRAS
• Long gene name
  RAS related
• Synonyms gene name
  • related RAS viral (r-ras) oncogene homolog
• Synonyms
  • R-Ras
• Synonyms name
  • Oncogene RRAS
• Locus
  19q13.33
• Discovery year
  2001-06-22
• Entrez gene record
  6237
• Pubmed identfication
  • 3098437
• RefSeq identity
  • NM_006270
• Classification
  • RAS type GTPase family
• VEGA ID
  OTTHUMG00000183247

Gene info

• Identity
  HGNC:9876
• Gene
  RASGRF2
• Long gene name
  Ras protein specific guanine nucleotide releasing factor 2
• Synonyms
  • GRF2
  • Ras-GRF2
• GenBank acession
  • AF023130
• Locus
  5q14.1
• Discovery year
  1996-12-20
• Entrez gene record
  5924
• RefSeq identity
  • NM_006909
• Classification
  • Pleckstrin homology domain containing
  • Dbl family Rho GEFs
• VEGA ID
  OTTHUMG00000119015

Gene info

• Identity
  HGNC:7227
• Gene
  MRAS
• Long gene name
  muscle RAS oncogene homolog
• Synonyms
  • M-RAs
  • R-RAS3
  • RRAS3
• GenBank acession
  • AF022080
• Locus
  3q22.3
• Discovery year
  1999-09-29
• Entrez gene record
  22808
• Pubmed identfication
  • 9400994
  • 10446149
• Classification
  • RAS type GTPase family
• VEGA ID
  OTTHUMG00000159888

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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