Rabbit Anti-Human PUMA (NT) Polyclonal: ATTO 565
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Catalog numberSPC-165D-A565
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Price:
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Size100 µg
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ImmunogenN-terminal amino acids of human PUMA
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Antibody s targetHuman PUMA
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Antibody s full descriptionRabbit Anti-Human PUMA (NT) Polyclonal
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Primary research fieldsCancer, Apoptosis
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameBBC3 Antibody, BCL2 binding component 3 Antibody, p53 up regulated modulator of apoptosis Antibody, PUMA/JFY1 Antibody
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Verified applicationsWB, IHC
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Raised inRabbit
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Antibody s reactivityHuman, Rat, Rainbow Trout (Oncorhynchus mykiss)
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Antibody s recommended dilutions for useWB (1:500), IHC (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromProtein A Purified
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Recommended buffer for storagePBS, 0.02% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects the N-terminal domain of PUMA ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAβ.
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Storage recommendations4°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysis2 µg/ml of SPC-165 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellMitochondrion
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextApoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
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Bibliography1. Nakano K., Vousden K.H. (2001) Mol Cell. 2001; 7(3): 683-94. 2. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. (2001) Mol Cell. 7(3): 673-82. 3. Han J., et al. (2001) Proc Natl Acad Sci U S A. 98(20): 11318-23.
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Released date1/Jun/2007
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NCBI numberNP_001120712.1
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Gene number27114
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Protein numberQ9BXH2
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PubMed number27872197|22736709
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Tested applications|Immunohistochemistry
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Tested reactivityRainbow Trout|Rat
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody (SPC-165). Tissue: breast carcinoma. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-165) at 1:100. | Western blot analysis of Human K562 cells lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody (SPC-165). Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-165) at 1:500. | Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody (SPC-165). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-165) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. Rabbit Anti-PUMA Antibody used in Immunohistochemistry (IHC) on Human breast carcinoma (SPC-165) | Rabbit Anti-PUMA Antibody used in Western blot (WB) on Human K562 cells lysates (SPC-165) | Rabbit Anti-PUMA Antibody used in Immunohistochemistry (IHC) on Human colon carcinoma (SPC-165)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolBBC3, HCAR2
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Short nameRabbit Anti- PUMA (NT) Polyclonal: ATTO 565
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 565
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman PUMA (NT) polyclonal: ATTO 565
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameBCL2 binding component 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-07-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- BCL2 homology region 3 (BH3) only
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namehydroxycarboxylic acid receptor 2
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Synonyms gene
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Synonyms gene name
- G protein-coupled receptor 109A
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-04-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Hydroxycarboxylic acid receptors
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data