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Immunogen
Full length protein, BC001304.1
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Antibody s target
Human Piccolo
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Antibody s full description
Rabbit Anti-Human Piccolo Polyclonal
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Primary research fields
Neuroscience, Cell Structure, Pre-Synaptic Markers
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ACZ Antibody, Aczonin Antibody, Brain-derived HLMN protein Antibody, DKFZp779G1236 Antibody, KIAA0559 Antibody, Multidomain presynaptic cytomatrix protein Antibody, PCLO Antibody, PCLO_HUMAN Antibody, Piccolo (presynaptic cytomatrix protein) Antibody, Piccolo mouse homolog of Pico Antibody, Presynaptic cytomatrix protein Antibody, Protein piccolo Antibody
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Verified applications
WB, IHC
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:400); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~550kDa. No cross-reactivity to Bassoon.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
1 µg/ml of SPC-197 was sufficient for detection of Piccolo in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Junction, Synapse
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).
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Bibliography
1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.
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Released date
22/Mar/2011
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NCBI number
NP_055325.2
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Gene number
27445
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Protein number
Q9Y6VO
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ. | Western blot analysis of Mouse, Rat brain cell lysates showing detection of Piccolo protein using Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197). Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197) at 1:1000. Rabbit Anti-Piccolo Antibody used in Immunohistochemistry (IHC) on Mouse Muscle (SPC-197) | Rabbit Anti-Piccolo Antibody used in Western blot (WB) on Mouse, Rat brain cell lysates (SPC-197)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
