Rabbit Anti-Human p16INK4a Polyclonal: ATTO 633
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Catalog numberSPC-1280D-A633
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Price:
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Size100 µl
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ImmunogenSynthesized peptide derived from human p12 INK antibody.
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Antibody s targetHuman p16INK4a
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Antibody s full descriptionRabbit Anti-Human p16INK4a Polyclonal
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Primary research fieldsCancer, Cell Cycle
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameCDKN2A Antibody, CDKN2 Antibody, MTS1 Antibody, Cyclin-dependent kinase inhibitor 2A Antibody, isoforms 1/2/3, Cyclin-dependent kinase 4 inhibitor A Antibody, CDK4I Antibody, p16-INK4a Antibody, Multiple tumor suppressor 1 Antibody
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Verified applicationsWB, ELISA
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Raised inRabbit
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Antibody s reactivityHuman, Mouse
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Antibody s recommended dilutions for useWB (1:1000), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects endogenous levels of total p16 INK protein.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-1280 was sufficient for detection of p16INK4a in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm, Nucleus
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Tissue specificityWidely expressed but not detected in brain or skeletal muscle.
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Scientific contextp16INK4a is a 16 kDa member of the CDKN2 cyclin-dependent kinase inhibitor family of molecules. It is widely expressed (although not in skeletal muscle) and serves as a negative regulator of cell proliferation. It does so by associating with CDK4 or 6, thereby blocking cyclin binding and subsequent Ser/Thr kinase activity.
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Bibliography1. Ohtani N., Yamakoshi K., Takahashi A., Hara E. (2004) J Med Invest. 51(3-4): 146-153.
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Released date6/Sep/2016
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NCBI numberNP_000068.1
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Gene number1029
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Protein numberP42771
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure legendWestern blot analysis of Human HeLa cell lysates showing detection of ~16kDa p16INK4a protein using Rabbit Anti-p16INK4a Polyclonal Antibody (SPC-1280). Lane 1: Human HeLa cells. Lane 2: Human HeLa cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-p16INK4a Polyclonal Antibody (SPC-1280) at 1:1000. Predicted/Observed Size: ~16kDa. Rabbit Anti-p16INK4a Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1280)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Short nameRabbit Anti- p16INK4a Polyclonal: ATTO 633
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeIgG
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LabelATTO 633
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman p16INK4a polyclonal: ATTO 633
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data