Rabbit Anti-Human NRF2 Polyclonal: Streptavidin

• Catalog number
  SPC-711D-STR
• Price
  Please ask
• Size
  100 µg

• Immunogen
  Synthetic peptide from the mid-protein of Human NRF2
• Antibody s target
  Human NRF2
• Antibody s full description
  Rabbit Anti-Human NRF2 Polyclonal
• Primary research fields
  Cell Signaling, Epigenetics and Nuclear Signalling, Transcription, Cardiovascular System, Heart, Cell Signaling, Metabolism, Mitochondrial Metabolism, Cell Signaling, Metabolism, Redox Metabolism, Metabolism and Oxidative Stress
• Antibody s category
  Polyclonal Antibodies
• Antibody s other name
  Nuclear factor erythroid 2-related factor 2 Antibody, Nrf 2 Antibody, Nuclear factor Antibody, NF2L2_HUMAN Antibody, Nfe2l2 Antibody, Nuclear factor erythroid 2 related factor 2 Antibody, nuclear factor erythroid 2 like 2 Antibody, Nuclear factor (erythroid derived 2) like 2 Antibody, NRF2 Antibody, like 2 Antibody, NF-E2-related factor 2 Antibody, NFE2-related factor 2 Antibody, erythroid derived 2, Nuclear factor, NFE2L2 Antibody, HEBP1 Antibody, NFE2 related factor 2 Antibody, NF E2 related factor 2 Antibody, Nuclear factor erythroid derived 2 like 2 Antibody, erythroid derived 2 Antibody
• Verified applications
  WB
• Raised in
  Rabbit
• Antibody s reactivity
  Human
• Antibody s recommended dilutions for use
  WB (1:1000); optimal dilutions for assays should be determined by the user.
• Antibody s purified from
  Peptide Affinity Purified
• Recommended buffer for storage
  PBS, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~67.8 kDa.
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Certificate of analysis
  A 1:1000 dilution of SPC-711 was sufficient for detection of NRF2 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Antibody in cell
  Cytoplasm, Cytosol, Nucleus
• Tissue specificity
  Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
• Scientific context
  See included datasheet or contact our support service.
• Bibliography
  Refer to PubMed
• Released date
  25/Oct/2016
• NCBI number
  NP_001138884.1
• Gene number
  4780
• Protein number
  Q16236
• PubMed number
  Refer to PubMed
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• Antibody s datasheet
  Contact our support service to receive datasheet or other technical documentation.
• Representative figure link
• Representative figure legend
  Western blot analysis of Human HeLa cell lysates showing detection of ~67.8 kDa NRF2 protein using Rabbit Anti-NRF2 Polyclonal Antibody (SPC-711). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NRF2 Polyclonal Antibody (SPC-711) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~67.8 kDa. Rabbit Anti-NRF2 Antibody used in Western blot (WB) on HeLa cell lysates (SPC-711)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availabilit
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  StressMark antibodies supplies antibodies that are for research of human proteins.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  NRF2   Streptavidin  
• Gene symbol
  NFE2L2, GABPA
• Short name
  Rabbit Anti- NRF2 Polyclonal: Streptavidin
• Technique
  anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  Immunoglobulin
• Label
  Streptavidin
• Species
  Human, Humans
• Alternative name
  production species: rabbit Antibody toHuman NRF2 polyclonal: biotinilated-binding streptavidin
• Alternative technique
  polyclonals, rabbit-anti, antibodies

Gene info

• Identity
  HGNC:7782
• Gene
  NFE2L2
• Long gene name
  NFE2 like bZIP transcription factor 2
• Synonyms gene name
  • nuclear factor (erythroid-derived 2)-like 2
  • nuclear factor, erythroid 2-like 2
  • nuclear factor, erythroid 2 like 2
• Synonyms
  • NRF2
  • NRF-2
• Synonyms name
  • NF-E2-related factor 2
• Locus
  2q31.2
• Discovery year
  1994-03-24
• Entrez gene record
  4780
• Pubmed identfication
  • 7937919
• RefSeq identity
  • NM_006164
• Classification
  • Basic leucine zipper proteins
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000133620

Gene info

• Identity
  HGNC:4071
• Gene
  GABPA
• Long gene name
  GA binding protein transcription factor subunit alpha
• Synonyms gene name
  • GA-binding protein transcription factor, alpha subunit (60kD)
  • GA binding protein transcription factor, alpha subunit 60kDa
  • GA binding protein transcription factor alpha subunit
• Synonyms
  • E4TF1A
  • NFT2
  • NRF2
  • E4TF1-60
  • NRF2A
• Synonyms name
  • human nuclear respiratory factor-2 subunit alpha
  • nuclear respiratory factor 2 alpha subunit
• Locus
  21q21.3
• Discovery year
  1994-01-18
• Entrez gene record
  2551
• Pubmed identfication
  • 8441384
• RefSeq identity
  • NM_002040
• Classification
  • ETS transcription factor family
• VEGA ID
  OTTHUMG00000078443

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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