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Immunogen
A synthesized peptide derived from human NF-kappaB p65
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Antibody s target
Human NFkB p65
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Antibody s full description
Rabbit Anti-Human NFkB p65 Polyclonal
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Primary research fields
Cancer, Apoptosis, Neuroscience, Neurodegeneration, Alzheimer’s Disease, Cell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, NFkB Pathway, Cardiovascular System, Atherosclerosis, Vascular Inflammation, Inflammatory Mediators, Cell Signaling, Metabolism
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
NFKB3 Antibody, Nuclear factor NF-kappa-B p65 subunit Antibody, RELA Antibody, TF65 Antibody, Transcription factor p65 Antibody, p65 Antibody
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Verified applications
WB, IHC, ICC/IF, IP
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~69kDa, endogenous levels of total NF-kappaB p65
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1279 was sufficient for detection of NFkB p65 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus, Cytoplasm
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Tissue specificity
Ubiquituous.
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Scientific context
NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (IL-1 & TNF, etc.), and viral infection, IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.
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Bibliography
1. Baeuerle P.A. and Henkel T. (1994) Annu Rev Immunol. 12: 141-79. 2. Baeuerle P.A. and Baltimore D. (1996) Cell. 87: 13-20. 3. Haskill S., et al. (1991) Cell. 65: 1281-9. 4. Thompson J.E., et al. (1995) Cell. 80: 573-82. 5. Whiteside S.T., et al. (1997) EMBO J. 16: 1413-26.
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Released date
6/Sep/2016
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NCBI number
NP_068810.3
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Gene number
5970
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Protein number
Q04206
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human HeLa cell lysates showing detection of ~65kDa NFkB p65 protein using Rabbit Anti-NFkB p65 Polyclonal Antibody (SPC-1279). Lane 1: Human HeLa treated with the immunizing peptide. Lane 2: Human HeLa. Primary Antibody: Rabbit Anti-NFkB p65 Polyclonal Antibody (SPC-1279) at 1:1000. Predicted/Observed Size: ~65kDa. Rabbit Anti-NFkB p65 antibody used in Western blot (WB) on HeLa cell lysates (SPC-1279)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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Gene
NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection (κ light chains are critical components of immunoglobulins). Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
