-
Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr1003 of human Met (AA1000-1006)
-
Antibody s target
Human Met (pTyr1003)
-
Antibody s full description
Rabbit Anti-Human Met (pTyr1003) Polyclonal
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
AUTS9 antibody, c met antibody, D249 antibody, Hepatocyte growth factor receptor antibody, HGF antibody, HGFR antibody, MET antibody, Met proto oncogene tyrosine kinase antibody, MET_HUMAN antibody, Oncogene MET antibody, Par4 antibody, Proto-oncogene c-Met antibody, RCCP2 antibody, Scatter factor receptor antibody, SF receptor antibody, Tyrosine-protein kinase Met antibody
-
Verified applications
WB, AM
-
Raised in
Rabbit
-
Antibody s reactivity
Human
-
Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
-
Antibody s concentration
See included datasheet or contact our support service
-
Antibody s specificity
Detects 155.527 kDa.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:250 dilution of SPC-1023 was sufficient for detection of Met (pTyr1003) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Membrane
-
Tissue specificity
Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain.
-
Scientific context
MET is a receptor-tyrosine kinase that is activated upon binding hepatocyte growth factor (HGF). MET/HGF signalling has been implicated in the generation and progression of a variety of tumours. The coexpression of wild type MET and HGF in the same cell (which generates an autocrine stimulatory loop) induces oncogenic transformation. All investigated naturally occurring MET mutants show increased tyrosine phosphorylation level and enhanced kinase activity
-
Bibliography
Refer to PubMed
-
Released date
29/Aug/2016
-
NCBI number
NP_000236
-
Gene number
4233
-
Protein number
P08581
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
Representative figure link
No Data Available
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus