Rabbit Anti-Human MAPKAPK5 (pThr186) Polyclonal: Biotin
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Catalog numberSPC-1017D-BI
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PricePlease ask
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Size100 µl
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ImmunogenA phospho-specific peptide corresponding to residues surrounding Thr186 of human MAPKAPK5 (AA183-190)
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Antibody s targetHuman MAPKAPK5 (pThr186)
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Antibody s full descriptionRabbit Anti-Human MAPKAPK5 (pThr186) Polyclonal
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameMAP kinase-activated protein kinase 5 antibody, MAPK-activated protein kinase 5 antibody, MAPK5_HUMAN antibody, MAPKAP kinase 5 antibody, MAPKAPK5 antibody, Mitogen activated protein kinase activated protein kinase 5 antibody, p38-regulated/activated protein kinase antibody, PRAK antibody
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Verified applicationsWB, AM
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Raised inRabbit
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Antibody s reactivityHuman
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Antibody s recommended dilutions for useWB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects 54.22 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:250 dilution of SPC-1017 was sufficient for detection of MAPKAPK5 (pThr186) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm, Nucleus
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Tissue specificityExpressed ubiquitously.
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Scientific contextSee included datasheet or contact our support service.
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BibliographyRefer to PubMed
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Released date29/Aug/2016
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NCBI numberNP_620777
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Gene number8550
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Protein numberQ8IW41
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure linkNo Data Available
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationBiotinylated
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolMAPKAPK5-AS1, MAPKAPK5
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Short nameRabbit Anti- MAPKAPK5 (pThr186) Polyclonal:
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelBiotin
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman MAPKAPK5 (pThr186) polyclonal: biotinilated
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameMAPKAPK5 antisense RNA 1
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Synonyms gene
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Synonyms gene name
- chromosome 12 open reading frame 47
- MAPKAPK5 antisense RNA 1 (non-protein coding)
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Synonyms
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Locus
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Discovery year2006-01-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameMAPK activated protein kinase 5
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Synonyms gene name
- mitogen-activated protein kinase-activated protein kinase 5
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-03-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MAPK activated protein kinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data