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Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser334 of human COT (AA331-337)
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Antibody s target
Human MAP3K8 (pSer334)
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Antibody s full description
Rabbit Anti-Human MAP3K8 (pSer334) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Cancer Osaka thyroid oncogene Antibody, CCOT Antibody, COT Antibody, EST Antibody, Ewing sarcoma transformant Antibody, M3K8_HUMAN Antibody, MEKK8 Antibody, Mitogen activated protein kinase kinase kinase 8 Antibody, Serine/threonine protein kinase cot Antibody, TPL 2 Antibody, Tumor progression locus 2 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 52.898 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-953 was sufficient for detection of MAP3K8 (pSer334) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
Expressed in several normal tissues and human tumor-derived cell lines.
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Scientific context
MAP3K8 is a protein serine/threonine kinase that can activate both MAPK and JNKK pathways. It is required for TLR4 activation of the MEK/ERK pathway. It can activate NF-kB1 by stimulating the proteolysis of its inhibitor, p105, and promote the production of TNF-alpha and IL-2 during T-cell activation. It has been shown to mediate Raf-inhibiting drug resistance in BRAF(V600E) cell lines. MAP3K8 may also play a role in the cell cycle.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_005195
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Gene number
1326
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Protein number
P41279
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus