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Immunogen
Synthesized peptide derived from C-terminal of human ki67.
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Antibody s target
Human Ki67
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Antibody s full description
Rabbit Anti-Human Ki67 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Antigen identified by monoclonal antibody Ki 67 antibody, Antigen KI-67 antibody, Antigen KI67 antibody, KI67_HUMAN antibody, KIA antibody, Marker of proliferation Ki-67 antibody, MIB 1 antibody, MIB antibody, MKI67 antibody, PPP1R105 antibody, Proliferation related Ki 67 antigen antibody, Protein phosphatase 1 regulatory subunit 105 antibody, RP11-380J17.2 antibody
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Verified applications
IHC, ICC/IF, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total ki67protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:100 dilution of SPC-1327 was sufficient for detection of Ki67 in human lung carcinoa tissue using Goat Anti-Rabbit IgG as the secondary antibody.
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Antibody in cell
Nucleus, Chromosome
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Antigen KI-67 also known as Ki-67 or MKI67 is a protein that in humans is encoded by the MKI67 gene. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore, it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. Due to its association with cell divison process, Ki-67 is routinely used as cellular proliferation marker of solid tumors as well as certain hematological malignancies, and a correlation has been demonstrated between Ki-67 index and the histopathological grade of cancers.
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Bibliography
1. Schonk DM. et al. (1989) Hum. Genet. 83 (3): 297–9. 2. Bullwinkel J. et al. (2006) J. Cell. Physiol. 206 (3): 624–35. 3. Rahmanzadeh R. et al. (2007) Cell Prolif. 40 (3): 422–30. 4. Scholzen T and Gerdes J (2000) J. Cell. Physiol. 182 (3): 311–22.
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Released date
6/Sep/2016
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NCBI number
NP_002408.3
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Gene number
4288
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Protein number
P46013
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327). Tissue: Cos7. Species: Monkey. Primary Antibody: Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-Ki67 Antibody used in Immunohistochemistry (IHC) on Lung Carcinoma Tissue (SPC-1327) | Rabbit Anti-Ki67 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cos7 (SPC-1327)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
