-
Immunogen
Synthetic peptide of human JAK2 (AA1118-1132)
-
Antibody s target
Human JAK2
-
Antibody s full description
Rabbit Anti-Human JAK2 Polyclonal
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
JAK 2 Antibody, JAK-2 Antibody, JAK2_HUMAN Antibody, Janus Activating Kinase 2 Antibody, JTK 10 Antibody, kinase Jak2 Antibody, THCYT3 Antibody, Tyrosine protein kinase JAK2 Antibody
-
Verified applications
WB, AM
-
Raised in
Rabbit
-
Antibody s reactivity
Human, Mouse
-
Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
-
Antibody s concentration
See included datasheet or contact our support service
-
Antibody s specificity
Detects 130.674 kDa.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:250 dilution of SPC-1149 was sufficient for detection of JAK2 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Endomembrane system, Nucleus
-
Tissue specificity
Expressed in blood, bone marrow and lymph node.
-
Scientific context
JAK2 is a non-receptor protein-tyrosine kinase. Involved in cellular responses to growth factors and cytokines such as IL-3, GM-CSF and erythropoietin. Phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates receptor bound STATs, which then form homo- or heterodimers and migrate to the nucleus to activate gene transcription.
-
Bibliography
Refer to PubMed
-
Released date
29/Aug/2016
-
NCBI number
NP_004963
-
Gene number
3717
-
Protein number
O60674
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
Representative figure link
No Data Available
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Conjugation
HRP Conjugated
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Gene target
-
Gene symbol
JAK2
-
Short name
Rabbit Anti- JAK2 Polyclonal:
-
Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit, Rabbits
-
Isotype
Immunoglobulin
-
Label
HRP
-
Species
Human, Humans
-
Alternative name
production species: rabbit Antibody toHuman Janus kinase 2 polyclonal: horseradish peroxidase
-
Alternative technique
polyclonals, rabbit-anti, antibodies
-
Alternative to gene target
Janus kinase 2, JTK10 and THCYT3, JAK2 and IDBG-47039 and ENSG00000096968 and 3717, transferase activity, nuclei, Jak2 and IDBG-156977 and ENSMUSG00000024789 and 16452, BT.25287 and IDBG-631361 and ENSBTAG00000012047 and 525246
-