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Immunogen
A synthesized peptide derived from human Integrin beta3
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Antibody s target
Human Integrin beta 3
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Antibody s full description
Rabbit Anti-Human Integrin beta 3 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
BDPLT16 antibody, BDPLT2 antibody, CD 61 antibody, CD61 antibody, CD61 antigen antibody, GP3A antibody, GPIIIa antibody, GT antibody, HPA 1 antibody, HPA 4 antibody, Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody, Integrin beta chain beta 3 antibody, Integrin beta-3 antibody, ITB3_HUMAN antibody, ITG B3 antibody, ITGB 3 antibody, ITGB3 antibody, NAIT antibody, Platelet fibrinogen receptor beta subunit antibody, Platelet fibrinogen receptor, beta subunit antibody, Platelet glycoprotein IIIa antibody, Platelet glycoprotein IIIa precursor antibody, Platelet membrane glycoprotein IIIa antibody, PTP antibody
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Verified applications
WB, IHC
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total Integrin beta3
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1326 was sufficient for detection of Integrin beta 3 in 10 µg of NIH/3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Membrane, Cell Junction, Cell Projection
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Integrin beta-3 (β3) or CD61 is a protein that in humans is encoded by the ITGB3 gene. CD61 is a cluster of differentiation found on thrombocytes. It associates with the integrin α_v chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin α_IIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of α_v/β_3 integrin or vitronectin receptor to CD31 (PECAM-1). The 2C9.G2 antibody reacts with mouse and rat CD61, and is able to block ligand binding.
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Bibliography
1. Sosnoski DM. et al. (1988) J Clin Invest 81 (6): 1993–8. 2. May, K. E. et al. (2011) Human Reproduction Update 17 (5): 637–653.
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Released date
6/Sep/2016
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NCBI number
NP_000203.2
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Gene number
3690
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Protein number
P05106
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~110kDa Integrin beta 3 protein using Rabbit Anti-Integrin beta 3 Polyclonal Antibody (SPC-1326). Lane 1: Mouse NIH/3T3 lysate treated with the immunizing peptide. Lane 2: Mouse NIH/3T3 lysate. Primary Antibody: Rabbit Anti-Integrin beta 3 Polyclonal Antibody (SPC-1326) at 1:1000. Predicted/Observed Size: ~110kDa. Rabbit Anti-Integrin beta 3 antibody used in Western blot (WB) on NIH/3T3 cell lysates (SPC-1326)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
