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Immunogen
Synthetic peptide from the mid-protein of Human HSF2
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Antibody s target
Human HSF2
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Antibody s full description
Rabbit Anti-Human HSF2 Polyclonal
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Primary research fields
Cell Signaling, Epigenetics and Nuclear Signalling, Transcription
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Heat shock factor protein 2 Antibody, Heat shock transcription factor 2 Antibody, HSTF 2 Antibody, HSTF2 Antibody, HSF 2 Antibody, HSF2 Antibody, HSF2_HUMAN Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Rat
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~90 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-712 was sufficient for detection of HSF2 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus
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Scientific context
See included datasheet or contact our support service.
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Bibliography
Refer to PubMed
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Released date
25/Oct/2016
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NCBI number
NP_001129036.1
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Gene number
3298
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Protein number
Q03933
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Rat Liver cell lysates showing detection of ~60.3 kDa HSF2 protein using Rabbit Anti-HSF2 Polyclonal Antibody (SPC-712). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSF2 Polyclonal Antibody (SPC-712) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~60.3 kDa. Rabbit Anti-HSF2 Antibody used in Western blot (WB) on Liver cell lysates (SPC-712)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugate
Peridinin chlorophyll (PerCP)
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
HSF2
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Short name
Rabbit Anti- HSF2 Polyclonal: PerCP
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Technique
anti-Human, Polyclonal, Rabbit, anti-, Percp, anti, antibody to, antibodies, antibodies against human proteins, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Rabbit Anti- HSF2 Polyclonal: PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMark antibodies. Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
PerCP
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman HSF2 polyclonal: PerCP
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Alternative technique
polyclonals, rabbit-anti, antibodies-percp, antibodies
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