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Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr356 of human GRK2 (AA353-359)
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Antibody s target
Human GRK2 (pTyr356)
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Antibody s full description
Rabbit Anti-Human GRK2 (pTyr356) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ADRBK1 Antibody, Adrenergic beta receptor kinase 1 Antibody, ARBK1_HUMAN Antibody, BARK Antibody, BARK1 Antibody, Beta adrenergic receptor kinase 1 Antibody, Beta ARK 1 Antibody, G protein coupled receptor kinase 2 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 79.574 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-922 was sufficient for detection of GRK2 (pTyr356) in 10 µg of human colon cancer cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Cell Membrane
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Tissue specificity
Expressed in peripheral blood leukocytes.
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Scientific context
G protein-coupled receptor kinases (GRKs) belong to a class of enzymes that regulate signal transduction. At least seven members of the GRK family have been identified. These include rhodopsin kinase (GRK 1), two forms of beta-adrenergic receptor kinase: GRK 2 (betaARK, betaARK1) and GRK 3 (betaARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_001610
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Gene number
156
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Protein number
P25098
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
GRK2
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Short name
Rabbit Anti- GRK2 (pTyr356) Polyclonal: FITC
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
FITC
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman GRK2 (pTyr356) polyclonal: fluorecein
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Alternative technique
polyclonals, rabbit-anti, antibodies, fluorescine
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