Rabbit Anti-Human Ghrelin Polyclonal: Biotin
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Catalog numberSPC-696D-BI
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PricePlease ask
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Size100 µg
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ImmunogenSynthetic peptide from the mid-protein of Human Ghrelin
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Antibody s targetHuman Ghrelin
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Antibody s full descriptionRabbit Anti-Human Ghrelin Polyclonal
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Primary research fieldsCardiovascular System, Blood, Cardiovascular System, Atherosclerosis, Diabetes Associated, Neuroscience, Cardiovascular System, Angiogenesis, Growth Factors
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameAppetite-regulating hormone Antibody, ghrl Antibody, Ghrelin 28 Antibody, Protein M46 Antibody, GHRL_HUMAN Antibody, GHRL Antibody, Ghrelin Antibody, Growth hormone-releasing peptide Antibody, Motilin-related peptide Antibody, Appetite regulating hormone Antibody, Growth hormone releasing peptide Antibody, Growth hormone secretagogue Antibody, Motilin related peptide Antibody, Ghrelin/obestatin prepropeptide Antibody, Obestatin Antibody, MTLRP Antibody, Ghrelin 27 Antibody, M46 protein Antibody
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Verified applicationsWB
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Raised inRabbit
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Antibody s reactivityRat
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Antibody s recommended dilutions for useWB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~13 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-696 was sufficient for detection of Ghrelin in 15 µg of rat Pancreas cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellSecreted
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Tissue specificityHighest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
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Scientific contextSee included datasheet or contact our support service.
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BibliographyRefer to PubMed
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Released date25/Oct/2016
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NCBI numberNP_001128413.1
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Gene number51738
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Protein numberQ9UBU3
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure legendWestern blot analysis of Rat Pancreas showing detection of ~12.9 kDa Ghrelin protein using Rabbit Anti-Ghrelin Polyclonal Antibody (SPC-696). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Ghrelin Polyclonal Antibody (SPC-696) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~12.9 kDa. Rabbit Anti-Ghrelin Antibody used in Western blot (WB) on Pancreas (SPC-696)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Ghrelin, GHRL gene, obestatin prepropeptide or lenomorelin from ghrelinergic cells in the gastrointestinal tract. GHRL is a neuropeptide in the central nervous system regulating appetite. Ghrelin is regulating the distribution and rate of use of body energy.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationBiotinylated
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolGHRL, GHRLOS, MBOAT4
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Short nameRabbit Anti- Ghrelin Polyclonal:
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelBiotin
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman Ghrelin polyclonal: biotinilated
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameghrelin and obestatin prepropeptide
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Synonyms gene name
- ghrelin, growth hormone secretagogue receptor ligand
- ghrelin/obestatin prepropeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-01-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Neuropeptides
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameghrelin opposite strand/antisense RNA
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Synonyms gene name
- ghrelin opposite strand RNA (non-protein coding)
- ghrelin opposite strand/antisense RNA (non-protein coding)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2007-11-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long non-coding RNAs with non-systematic symbols
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemembrane bound O-acyltransferase domain containing 4
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Synonyms gene
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Synonyms gene name
- O-acyltransferase (membrane bound) domain containing 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-09-27
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Entrez gene record
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Pubmed identfication
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Classification
- Membrane bound O-acyltransferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data