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Immunogen
Synthetic peptide from the C-terminal of Human GABARAPL2
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Antibody s target
Human GABARAPL2
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Antibody s full description
Rabbit Anti-Human GABARAPL2 Polyclonal
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Primary research fields
Cell Signaling, Protein Trafficking, Vesicle Transport, Cancer, Autophagy, Cell Signaling, Metabolism, Metabolism processes
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ATG8 Antibody, FLC3A Antibody, General protein transport factor p16 Antibody, GEF-2 Antibody, GABARAPL2 Antibody, GBRL2_HUMAN Antibody, GEF2 Antibody, GABA(A) receptor-associated protein-like 2 Antibody, MAP1 light chain 3-related protein Antibody, Golgi-associated ATPase enhancer of 16 kDa Antibody, Gabarapl2 Antibody, GATE-16 Antibody, Gamma-aminobutyric acid receptor-associated protein-like 2 Antibody, Ganglioside expression factor 2 Antibody, ATG8C Antibody, GATE16 Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Human, Rat
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~14 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-624 was sufficient for detection of GABARAPL2 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Golgi Apparatus, Cytoplasmic Vesicle, Autophagosome
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Tissue specificity
Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.
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Scientific context
See included datasheet or contact our support service.
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Bibliography
Refer to PubMed
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Released date
25/Oct/2016
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NCBI number
NP_009216.1
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Gene number
11345
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Protein number
P60520
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Rat Liver cell lysates showing detection of ~14 kDa GABARAPL2 protein using Rabbit Anti-GABARAPL2 Polyclonal Antibody (SPC-624). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL2 Polyclonal Antibody (SPC-624) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~14 kDa. Rabbit Anti-GABARAPL2 Antibody used in Western blot (WB) on Liver cell lysates (SPC-624)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus