Rabbit Anti-Human FUNDC1 Polyclonal: ATTO 565

• Catalog number
  SPC-638D-A565
• Price
  Please ask
• Size
  100 µg

• Immunogen
  Synthetic peptide from the N-terminal of Human FUNDC1
• Antibody s target
  Human FUNDC1
• Antibody s full description
  Rabbit Anti-Human FUNDC1 Polyclonal
• Primary research fields
  Cell Signaling, Metabolism, Metabolism processes, Hypoxia, Cancer, Autophagy
• Antibody s category
  Polyclonal Antibodies
• Antibody s other name
  FUNDC1 Antibody, FUN14 domain-containing protein 1 Antibody
• Verified applications
  WB
• Raised in
  Rabbit
• Antibody s reactivity
  Mouse, Rat
• Antibody s recommended dilutions for use
  WB (1:1000); optimal dilutions for assays should be determined by the user.
• Antibody s purified from
  Peptide Affinity Purified
• Recommended buffer for storage
  PBS, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~17 kDa.
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Certificate of analysis
  A 1:1000 dilution of SPC-638 was sufficient for detection of FUNDC1 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Antibody in cell
  Mitochondrion Outer Membrane, Multi-Pass Membrane Protein
• Tissue specificity
  Widely expressed.
• Scientific context
  See included datasheet or contact our support service.
• Bibliography
  Refer to PubMed
• Released date
  25/Oct/2016
• NCBI number
  NP_776155.1
• Gene number
  139341
• Protein number
  Q8IVP5
• PubMed number
  Refer to PubMed
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• Antibody s datasheet
  Contact our support service to receive datasheet or other technical documentation.
• Representative figure link
• Representative figure legend
  Western blot analysis of Mouse, Rat Liver cell lysates showing detection of ~17.2 kDa FUNDC1 protein using Rabbit Anti-FUNDC1 Polyclonal Antibody (SPC-638). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Lane 3: Rat Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody (SPC-638) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~17.2 kDa. Rabbit Anti-FUNDC1 Antibody used in Western blot (WB) on Liver cell lysates (SPC-638)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availabilit
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  StressMark antibodies supplies antibodies that are for research of human proteins.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  FUNDC1   ATTO   565  
• Gene symbol
  FUNDC1
• Short name
  Rabbit Anti- FUNDC1 Polyclonal: ATTO 565
• Technique
  anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  Immunoglobulin
• Label
  ATTO 565
• Species
  Human, Humans
• Alternative name
  production species: rabbit Antibody toHuman FUNDC1 polyclonal: ATTO 565
• Alternative technique
  polyclonals, rabbit-anti, antibodies

Gene info

• Identity
  HGNC:28746
• Gene
  FUNDC1
• Long gene name
  FUN14 domain containing 1
• Synonyms
  • MGC51029
• GenBank acession
  • BC042813
• Locus
  Xp11.3
• Discovery year
  2004-03-16
• Entrez gene record
  139341
• Pubmed identfication
  • 22267086
• RefSeq identity
  • NM_173794
• VEGA ID
  OTTHUMG00000021399
• Locus Specific Databases
  • Global Variome shared LOVD

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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