Rabbit Anti-Human Cpn10 Polyclonal: FITC

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• Catalog number
  SPC-193D-FITC
• Price:

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• Size
  100 µg

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• Immunogen
  Human Cpn10 peptide AA 91-101
• Antibody s target
  Human Cpn10
• Antibody s full description
  Rabbit Anti-Human Cpn10 Polyclonal
• Primary research fields
  Cancer, Heat Shock
• Antibody s category
  Polyclonal Antibodies
• Antibody s other name
  10kDa Chaperonin Antibody, Chaperonin 10 Antibody, Cpn 10 Antibody, EPF Antibody, GROES Antibody, Heat Shock 10kD protein1 Antibody, HSP10 Antibody, HSPE1 Antibody
• Verified applications
  WB, IHC, ICC/IF, IP, ELISA
• Raised in
  Rabbit
• Antibody s reactivity
  Human, Mouse, Rat, Bovine, Dog, Guinea Pig (Cavia porcellus), Pig, Rabbit, Sheep, African clawed frog (Xenopus laevis)
• Antibody s recommended dilutions for use
  WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
• Antibody s purified from
  Protein A Purified
• Recommended buffer for storage
  PBS pH7.3, 0.02% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~ 10kDa.
• Storage recommendations
  4°C
• Shipping recommendations
  Blue Ice or 4°C
• Certificate of analysis
  1 µg/ml of SPC-193 was sufficient for detection of Cpn10 in 10 µg of Hela lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Antibody in cell
  Mitochondrion, Mitochondrion Matrix
• Tissue specificity
  See included datasheet or contact our support service.
• Scientific context
  Chaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10kDa (HSP10s). Cpn10 acts as a co-chaperone and interacts with the HSP60 family to promote proper folding of polypeptides. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process (1). Cpn10 has been located in human platelets, but is also present in human maternal serum (2, 3). It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development. This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy (4).
• Bibliography
  1. Velez-Granell C.S., et al. (1994) J of Cell Science. 107(3): 539-549. 2. Morton H., Hegh V., and Clunie G.J.A. (1974) Nature (London) 249: 459-460. 3. Cavanagh A.C., and Morton H. (1994) Eur. J. Biochem. 222: 551-560. 4. Minto M., et al. (1998) Molecular Cell Research. 1403 (2): 151-157.
• Released date
  1/Dec/2009
• NCBI number
  NP_002148.1
• Gene number
  3336
• Protein number
  P61604
• PubMed number
  Refer to PubMed
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• Antibody s datasheet
  Contact our support service to receive datasheet or other technical documentation.
• Representative figure link
  more | details
• Representative figure legend
  Immunohistochemistry analysis using Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis.. | Western blot analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. Rabbit Anti-Cpn10 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-193) | Rabbit Anti-Cpn10 Antibody used in Western blot (WB) on Rat brain cell lysates (SPC-193)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availabilit
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  StressMark antibodies supplies antibodies that are for research of human proteins.
• Properties
  This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Anti-FITC Antibody
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

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• Gene target
  Cpn10  
• Gene symbol
  HSPE1
• Short name
  Rabbit Anti- Cpn10 Polyclonal: FITC
• Technique
  anti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  Immunoglobulin
• Label
  FITC
• Species
  Human, Humans
• Alternative name
  production species: rabbit Antibody toHuman Cpn10 polyclonal: fluorecein
• Alternative technique
  polyclonals, rabbit-anti, antibodies, fluorescine

Gene info

• Identity
  HGNC:5269
• Gene
  HSPE1
• Long gene name
  heat shock protein family E (Hsp10) member 1
• Synonyms gene name
  • heat shock 10kD protein 1 (chaperonin 10)
  • heat shock 10kDa protein 1
• Synonyms
  • CPN10
  • GroES
  • HSP10
  • EPF
• Synonyms name
  • chaperonin 10
  • early pregnancy factor
• GenBank acession
  • AF109872
• Locus
  2q33.1
• Discovery year
  1994-11-16
• Entrez gene record
  3336
• Pubmed identfication
  • 7914093
  • 7698325
• RefSeq identity
  • NM_002157
• Classification
  • Chaperonins
• VEGA ID
  OTTHUMG00000132749

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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