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Immunogen
Synthetic peptide of Human CCR2
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Antibody s target
Human CCR2
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Antibody s full description
Rabbit Anti-Human CCR2 Polyclonal
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Primary research fields
Immunology, Innate Immunity, Macrophage, Immunology, Innate Immunity, Chemokines, Cell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, STAT, Cardiovascular System, Atherosclerosis, Vascular Inflammation, Leukocyte Recruitment, Cancer
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
CKR2 Antibody, C-C chemokine receptor type 2 Antibody, Monocyte Chemotactic Protein 1 Receptor Antibody, CC chemokine receptor type 2 Antibody, CCR1L Antibody, CCCKR2 Antibody, CD192 Antibody, MCP-1-R Antibody, MCP1 RECEPTOR Antibody, CKR2B Antibody, C-C CKR-2 Antibody, CC CKR 2 Antibody, CD_antigen: CD192 Antibody, CC-CKR-2 Antibody, CCR5L Antibody, C C chemokine receptor type 2 Antibody, CD192 antigen Antibody, CKR2A Antibody, CCR2 Antibody, Monocyte chemoattractant protein 1 receptor Antibody, CCR 2 Antibody, CCR2B Antibody, C C CKR 2 Antibody, CCR-2 Antibody, CMKBR2 Antibody, MCP 1 R Antibody, CCR2A Antibody, Chemokine CC Motif Receptor 2 Antibody, Chemokine C C motif receptor 2 Antibody, CCR2_HUMAN Antibody, MCP1R Antibody, CKR 2 Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Rat
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~42 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-704 was sufficient for detection of CCR2 in 15 µg of rat brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Membrane, Multi-Pass Membrane Protein
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Scientific context
See included datasheet or contact our support service.
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Bibliography
Refer to PubMed
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Released date
25/Oct/2016
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NCBI number
NP_001116513.2
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Gene number
729230
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Protein number
P41597
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Rat Brain cell lysates showing detection of ~41.9 kDa CCR2 protein using Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~41.9 kDa. Rabbit Anti-CCR2 Antibody used in Western blot (WB) on Brain cell lysates (SPC-704)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugate
Peridinin chlorophyll (PerCP)
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
CCR2
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Short name
Rabbit Anti- CCR2 Polyclonal: PerCP
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Technique
anti-Human, Polyclonal, Rabbit, anti-, Percp, anti, antibody to, antibodies, antibodies against human proteins, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Rabbit Anti- CCR2 Polyclonal: PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMark antibodies. Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
PerCP
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman chemokine (C-C motif) receptor 2 polyclonal: PerCP
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Alternative technique
polyclonals, rabbit-anti, antibodies-percp, antibodies
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Alternative to gene target
chemokine (C-C motif) receptor 2, CCR2 and IDBG-30182 and ENSG00000121807 and 729230, protein homodimerization activity, Plasma membranes, BT.63814 and IDBG-647810 and ENSBTAG00000013586 and 539002
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