Rabbit Anti-Human Aurora A Polyclonal: HRP
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Catalog numberSPC-1106D-HRP
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PricePlease ask
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Size100 µl
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ImmunogenSynthetic peptide of human Aurora A (AA11-25)
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Antibody s targetHuman Aurora A
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Antibody s full descriptionRabbit Anti-Human Aurora A Polyclonal
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameAurora 2 Antibody, AIK Antibody, ARK1 Antibody, BTAK Antibody
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Verified applicationsWB, AM
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Raised inRabbit
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Antibody s reactivityHuman, Mouse
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Antibody s recommended dilutions for useWB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects ~48kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:250 dilution of SPC-1106 was sufficient for detection of Aurora A in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm, Cytoskeleton
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Tissue specificityHighly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
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Scientific contextAurora A is part of the larger AURA family of serine/threonine kinases and is a key regulator of mitotic cell division. It is involved in regulating microtubule formation and the assembly of the mitotic spindle responsible for chromosome segragation during mitosis. AURA activity is cell cycle dependent with the greatest activity occuring during mitosis. The overexpression of AURA has been well documented in many malignant cancers including breast, ovarian, colon, prostate and neuroblastomas. Overexpression of AURA appears to override checkpoints in cell cycle progression leading to increased cell division and proliferation.
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BibliographyRefer to PubMed
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Released date29/Aug/2016
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NCBI numberNP_003591
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Gene number6790
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Protein numberO14965
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure linkNo Data Available
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationHRP Conjugated
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolBORA, AURKAP2, AURKAIP1
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Short nameRabbit Anti- Aurora A Polyclonal:
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelHRP
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman Aurora A polyclonal: horseradish peroxidase
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameBORA aurora kinase A activator
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Synonyms gene
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Synonyms gene name
- chromosome 13 open reading frame 34
- bora, aurora kinase A activator
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-08-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameaurora kinase A pseudogene 2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2003-07-21
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Entrez gene record
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameaurora kinase A interacting protein 1
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Synonyms
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Locus
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Discovery year2005-07-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data