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Clone
9H6
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Immunogen
Synthetic β-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
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Antibody s full description
Mouse Anti- O-GlcNAc Monoclonal IgM Antibody, Clone: 9H6: PerCP
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
β-O-Linked N-Acetylglucosamine Antibody, O-Linked N-Acetylglucosamine Antibody, O-GlcNAc Antibody, Beta-O-Linked N-Acetylglucosamine Antibody, N-Acetylglucosamine Antibody, GlcNac Antibody, N-Acetylglucosamine Antibody, 2-(Acetylamino)-2-deoxy-D-glucose Antibody, N-acetyl-D-glucosamine Antibody, NAG Antibody, β-D-(Acetylamino)-2-deoxy-glucopyranose Antibody, beta-N-Acetylglucosamine Antibody, N-Acetylglucosamine-modified protein Antibody, β-N-Acetylglucosamine Antibody
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Raised in
Mouse
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Antibody s target
O-GlcNAc
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Primary research fields
Cell Signaling, Post Translational Modifications, Glycation
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Brandname
MOLECULAR SIGNATURE®
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Antibodies applications
WB, ICC/IF, ELISA
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Antibody s reactivity
Species Independent
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Antibody s dilutions
WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
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Purity
PEG Purified
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Antibody buffer for storage
PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Specific for β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:1000 dilution of SMC-502 was sufficient for detection of O-GlcNAc in 2 µg of O-GlcNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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Antibody in cell
See included datasheet or contact our support service
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Tissue specificity
See included datasheet or contact our support service
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Released date
23-Mar-2017
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
Refer to Swiss-Prot
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6 (SMC-502). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody (SMC-502) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250: 1:1000 for 30-60 min at RT. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. Western Blot analysis of GlcNAc-BSA Conjugate showing detection of 67 kDa GlcNAc-BSA using Mouse Anti-GlcNAc Monoclonal Antibody, Clone 9H6 (SMC-502). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: GlcNAc-BSA. Lane 4: GalNAc-BSA. Lane 5: Galactose-BSA. Lane 6: Glucose-BSA. Lane 7: Citrulline-BSA. Load: 2.0 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-GlcNAc Monoclonal Antibody (SMC-502) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. Mouse Anti-O-GlcNAc Antibody [9H6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Embryonic kidney cells (HEK293) (SMC-502) Mouse Anti-O-GlcNAc Antibody [9H6] used in Western Blot (WB) on GlcNAc-BSA Conjugate (SMC-502)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
