Mouse Anti-Rat SHANK3 Monoclonal IgG2b, Clone S69-46
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Catalog numberSMC-336D
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PricePlease ask
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Size100 µg
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CloneS69-46
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ImmunogenSynthetic peptide amino acids 840-857 of rat Shank3
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Antibody s full descriptionMouse Anti-Rat SHANK3 Monoclonal IgG2b Antibody, Clone: S69-46: Unconjugated
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameAI841104 antibody, DEL22q13.3 antibody, KIAA1650 antibody, Proline rich synapse associated protein 2 antibody, Proline-rich synapse-associated protein 2 antibody, ProSAP2 antibody, PSAP2 antibody, SH3 and multiple ankyrin repeat domains 3 antibody, SH3 and multiple ankyrin repeat domains protein 3 antibody SH3/ankyrin domain gene 3 antibody, SHAN3_HUMAN antibody, Shank postsynaptic density protein antibody, Shank3 antibody, Shank3b antibody, SPANK 2 antibody, SPANK2 antibody
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Raised inMouse
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Antibody s targetSHANK3
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Primary research fieldsNeuroscience, Cell Structure, Post-Synaptic Markers
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~190kDa. No cross-reactivity against Shank1 or Shank2.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-336 was sufficient for detection of Shank3 in 10 µg COS cell lysate transiently transfected with Shank3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextShank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are also crucial in receptor tyrosine kinase signaling; specifically, Shank3 can mediate Erk-MAPK and P13K signaling which is crucial for tubule formation (2). Shank3 is also one of the latest genes to be associated with autism. A mutation of a single copy of Shank3 on chromosome 22q13 can result in language and/or social communication disorders (3).
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Bibliography1. Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856. 2. Schuetz G., et al. (2004) JCB. 167(5): 645-952. 3. Durand C.M., et al. (2007) Nature Genetics. 39: 25-27.
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Released date13-Apr-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_067708.1
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Gene number59312
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Protein numberQ9JLU4
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (SMC-336). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (SMC-336) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Early stages of filaggrin-like and dermal staining. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (SMC-336). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (SMC-336) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Borderline positive. Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (SMC-336). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (SMC-336) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-SHANK3 Antibody [S69-46] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-336) Mouse Anti-SHANK3 Antibody [S69-46] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-336) Mouse Anti-SHANK3 Antibody [S69-46] used in Western Blot (WB) on Rat brain membrane lysate (SMC-336)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolIGLV7-46, IGHV1-46, IGHVII-46-1, SNORD115-46, RNU6-46P, SHANK3, LAMP5, OR7E110P
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Short nameMouse Anti- SHANK3 Monoclonal IgG2b, Clone S69-46
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2b, IgG2b
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LabelUnconjugated
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat SHANK3 monoclonal IgG2b, clonality S69-46
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Alternative techniquemurine, antibodies
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Clone nameS69-46
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 7-46
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Synonyms gene name
- immunoglobulin lambda variable 7-46 (gene/pseudogene)
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 1-46
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-46-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-46-1
- immunoglobulin heavy variable (II)-46-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-46
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Synonyms
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Locus
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Discovery year2011-08-22
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Entrez gene record
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 46, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 46
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Locus
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Discovery year2008-06-10
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameSH3 and multiple ankyrin repeat domains 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-02-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PDZ domain containing
- Sterile alpha motif domain containing
- Ankyrin repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelysosomal associated membrane protein family member 5
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Synonyms gene
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Synonyms gene name
- chromosome 20 open reading frame 103
- lysosomal-associated membrane protein family, member 5
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Lysosome associated membrane proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 7 subfamily E member 110 pseudogene
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Synonyms gene
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Synonyms gene name
- olfactory receptor, family 7, subfamily E, member 110 pseudogene
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Synonyms
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Locus
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Discovery year2001-03-28
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Entrez gene record
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Classification
- Olfactory receptors, family 7
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data