Mouse Anti-Rat NPAS4 Monoclonal IgG1, Clone S408-79
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Catalog numberSMC-495D-STR
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PricePlease ask
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Size100 µg
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CloneS408-79
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ImmunogenFusion protein amino acids 597-802 (C-terminus) of rat Npas4.
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Antibody s full descriptionMouse Anti-Rat NPAS4 Monoclonal IgG1 Antibody, Clone: S408-79: Streptavidin
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameNeuronal PAS domain-containing protein 4 Antibody, Neuronal PAS4 Antibody, Class E basic helix-loop-helix protein 79 Antibody, HLH-PAS transcription factor NXF Antibody, PAS domain-containing protein 10 Antibody, Limbic-enhanced PAS protein Antibody, bHLHe79 Antibody, PASD10 Antibody and LE-PAS Antibody
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Raised inMouse
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Antibody s targetNPAS4
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Primary research fieldsCell Signaling, Epigenetics, Neuroscience
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityMouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~90kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:100 dilution of SMC-495 was sufficient for detection of NPAS4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellNucleus
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextNPAS4 belongs to the Per-Arnt-Sim family of neuronal specific transciption factors, all involved in the development and maintenance of learning and memory pathways (1). NPAS4 is a transcription factor that regulates the formation and maintenance of inhibitory synapses, and can activate the CNS midline enhancer element and the expression of the drebrin gene. Deregulation has been to be associated with developmental disorders such as schizophrenia and autism (2).
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Bibliography1. Zhaohui Z., et al. (2014) Exp Ther Med. 7(4): 1045-1049. 2. Coutellier L., et al. (2012) Plos One. 7(9): e46604.
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Released date10-Mar-2015
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_705890.1
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Gene number266734
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Protein numberQ8CJH6
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Antibody s datasheetContact our support service
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Representative figure legendWestern Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79 (SMC-495). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody (SMC-495) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa. Mouse Anti-NPAS4 Antibody [S408-79] used in Western Blot (WB) on Rat Brain (SMC-495)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolIGHV3-79, CCDC73, RBMX2, RNU6-79P, NPAS4
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Short nameMouse Anti- NPAS4 Monoclonal IgG1, Clone S408-79
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelStreptavidin
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat NPAS4 monoclonal IgG1, clonality S408-79
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Alternative techniquemurine, antibodies
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Clone nameS408-79
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-79 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-79
- immunoglobulin heavy variable 3-79 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecoiled-coil domain containing 73
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-02-11
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA binding motif protein X-linked 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-06-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RNA binding motif containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 79, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 79
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Locus
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Discovery year2011-08-08
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameneuronal PAS domain protein 4
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Basic helix-loop-helix proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data