Mouse Anti-Rat NPAS4 Monoclonal IgG1, Clone S408-79

• Catalog number
  SMC-495D-A565
• Price
  Please ask
• Size
  100 µg

• Clone
  S408-79
• Immunogen
  Fusion protein amino acids 597-802 (C-terminus) of rat Npas4.
• Antibody s full description
  Mouse Anti-Rat NPAS4 Monoclonal IgG1 Antibody, Clone: S408-79: ATTO 565
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  Neuronal PAS domain-containing protein 4 Antibody, Neuronal PAS4 Antibody, Class E basic helix-loop-helix protein 79 Antibody, HLH-PAS transcription factor NXF Antibody, PAS domain-containing protein 10 Antibody, Limbic-enhanced PAS protein Antibody, bHLHe79 Antibody, PASD10 Antibody and LE-PAS Antibody
• Raised in
  Mouse
• Antibody s target
  NPAS4
• Primary research fields
  Cell Signaling, Epigenetics, Neuroscience
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF
• Antibody s reactivity
  Mouse, Rat
• Antibody s dilutions
  WB (1:1000); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~90kDa.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  A 1:100 dilution of SMC-495 was sufficient for detection of NPAS4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Nucleus
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  NPAS4 belongs to the Per-Arnt-Sim family of neuronal specific transciption factors, all involved in the development and maintenance of learning and memory pathways (1). NPAS4 is a transcription factor that regulates the formation and maintenance of inhibitory synapses, and can activate the CNS midline enhancer element and the expression of the drebrin gene. Deregulation has been to be associated with developmental disorders such as schizophrenia and autism (2).
• Bibliography
  1. Zhaohui Z., et al. (2014) Exp Ther Med. 7(4): 1045-1049. 2. Coutellier L., et al. (2012) Plos One. 7(9): e46604.
• Released date
  10-Mar-2015
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_705890.1
• Gene number
  266734
• Protein number
  Q8CJH6
• Antibody s datasheet
  Contact our support service
• Representative figure link
• Representative figure legend
  Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79 (SMC-495). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody (SMC-495) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa. Mouse Anti-NPAS4 Antibody [S408-79] used in Western Blot (WB) on Rat Brain (SMC-495)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus

• Gene target
  NPAS4   Monoclonal   IgG1   S408-79  
• Gene symbol
  IGHV3-79, CCDC73, RBMX2, RNU6-79P, NPAS4
• Short name
  Mouse Anti- NPAS4 Monoclonal IgG1, Clone S408-79
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG1, IgG1
• Label
  ATTO 565
• Species
  Rat, Mouses, Rats
• Alternative name
  Mouse Antibody toRat NPAS4 monoclonal IgG1, clonality S408-79
• Alternative technique
  murine, antibodies
• Clone name
  S408-79

Gene info

• Identity
  HGNC:5627
• Gene
  IGHV3-79
• Long gene name
  immunoglobulin heavy variable 3-79 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 3-79
  • immunoglobulin heavy variable 3-79 pseudogene
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28405
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151871

Gene info

• Identity
  HGNC:23261
• Gene
  CCDC73
• Long gene name
  coiled-coil domain containing 73
• Synonyms
  • NY-SAR-79
• GenBank acession
  • AK128159
• Locus
  11p13
• Discovery year
  2006-02-11
• Entrez gene record
  493860
• RefSeq identity
  • NM_001008391
• VEGA ID
  OTTHUMG00000166279

Gene info

• Identity
  HGNC:24282
• Gene
  RBMX2
• Long gene name
  RNA binding motif protein X-linked 2
• Synonyms
  • CGI-79
  • Snu17
• GenBank acession
  • AF078865
• Locus
  Xq26.1
• Discovery year
  2004-06-02
• Entrez gene record
  51634
• Pubmed identfication
  • 10810093
• RefSeq identity
  • NM_016024
• Classification
  • RNA binding motif containing
• VEGA ID
  OTTHUMG00000022395

Gene info

• Identity
  HGNC:42569
• Gene
  RNU6-79P
• Long gene name
  RNA, U6 small nuclear 79, pseudogene
• Synonyms gene
  • RNU6-79
• Synonyms gene name
  • RNA, U6 small nuclear 79
• Locus
  13q22.1
• Discovery year
  2011-08-08
• Entrez gene record
  100873779
• RefSeq identity
  • NR_046945

Gene info

• Identity
  HGNC:18983
• Gene
  NPAS4
• Long gene name
  neuronal PAS domain protein 4
• Synonyms
  • PASD10
  • NXF
  • Le-PAS
  • bHLHe79
• GenBank acession
  • AB049469
• Locus
  11q13.2
• Discovery year
  2005-09-26
• Entrez gene record
  266743
• Pubmed identfication
  • 14701734
• RefSeq identity
  • NM_178864
• Classification
  • Basic helix-loop-helix proteins
• VEGA ID
  OTTHUMG00000167045

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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