Mouse Anti-Rat Nav beta 3 Monoclonal IgG2B, Clone S396-29

• Catalog number
  SMC-490D-RPE
• Price
  Please ask
• Size
  100 µg

• Clone
  S396-29
• Immunogen
  Fusion protein amino acids 1-215 (full-length) of rat NavBeta3
• Antibody s full description
  Mouse Anti-Rat NaVbeta3 Monoclonal IgG2B Antibody, Clone: S396-29: RPE
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  Sodium channel subunit beta-3 Antibody, Scn3b Antibody, KIAA1158 Antibody
• Raised in
  Mouse
• Antibody s target
  NaVbeta3
• Primary research fields
  Neuroscience, Ion Channels, Sodium Channels, Voltage-Gated Sodium Channels
• Brandname
  None
• Antibodies applications
  WB, ICC/IF
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~40kDa. Does not cross-reat with NavBeta1, Navbeta2, or Navbeta4.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  A 1:100 dilution of SMC-490 was sufficient for detection of NavBeta3 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  Nav Beta 3 (SCN3B) belongs to the voltage-gated sodium channel group, and modulates channel gating kinetics. It inactivates the sodium channel opening more slowly than its Beta1 subunit. It is also unique in causing persistent sodium currents which are thought to amplify summation of synaptic imputs which is likely to increase the excitability of specific neurons to their individual inputs (2). Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons (1, 2). Defects in SCN3B are the cause of Brugada syndrome (3).
• Bibliography
  1. Genes and mapped phenotypes. (n.d.). Retrieved March 26, 2015, from more 2. Qu Y., et al. (2001) Mol Cell Neurosci. 18(5): 570-580. 3. Ishikawa T., et al. (2013) Circ J. 77(4): 956-967.
• Released date
  10-Mar-2015
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_620797.1
• Gene number
  245956
• Protein number
  Q9JK00
• Antibody s datasheet
  Contact our support service
• Representative figure link
  details | see here
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav beta 3 Monoclonal Antibody, Clone S396-29 (SMC-490). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Nav beta 3 Monoclonal Antibody (SMC-490) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Nav beta 3 Antibody (D) Composite. Western Blot analysis of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein using Mouse Anti-Nav Beta 3 Monoclonal Antibody, Clone S396-29 (SMC-490). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 µg. Primary Antibody: Mouse Anti-Nav Beta 3 Monoclonal Antibody (SMC-490) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~40 kDa. Mouse Anti-Nav beta 3 Antibody [S396-29] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-490) Mouse Anti-Nav Beta 3 Antibody [S396-29] used in Western Blot (WB) on Mouse Brain (SMC-490)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus

• Gene target
  Nav   beta   Monoclonal   IgG2B   S396-29  
• Gene symbol
  SNORD114-29, SNORD115-29, SNORD116-29, IGLV3-29, IGHV3-29, IGKV2-29, IGKV2D-29, ERVK-29, ERVW-29, RNVU1-29
• Short name
  Mouse Anti- Nav beta 3 Monoclonal IgG2B, Clone S396-29
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG2B, IgG2b
• Label
  RPE
• Species
  Rat, Mouses, Rats
• Alternative name
  Mouse Antibody toRat Nav b 3 monoclonal IgG2B, clonality S396-29
• Alternative technique
  murine, antibodies
• Clone name
  S396-29

Gene info

• Identity
  HGNC:33017
• Gene
  SNORD114-29
• Long gene name
  small nucleolar RNA, C/D box 114-29
• Synonyms
  • 14q(II-29)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767610
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003222.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33048
• Gene
  SNORD115-29
• Long gene name
  small nucleolar RNA, C/D box 115-29
• Synonyms
  • HBII-52-29
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033803
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003344.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33348
• Gene
  SNORD116-29
• Long gene name
  small nucleolar RNA, C/D box 116-29
• Synonyms
  • HBII-85-29
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-16
• Entrez gene record
  100033821
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003360
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5911
• Gene
  IGLV3-29
• Long gene name
  immunoglobulin lambda variable 3-29 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable 3-29
• GenBank acession
  • Z73644
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28790
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151168

Gene info

• Identity
  HGNC:5590
• Gene
  IGHV3-29
• Long gene name
  immunoglobulin heavy variable 3-29 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 3-29
  • immunoglobulin heavy variable 3-29 pseudogene
• GenBank acession
  • M99662
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28440
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000188328

Gene info

• Identity
  HGNC:5784
• Gene
  IGKV2-29
• Long gene name
  immunoglobulin kappa variable 2-29
• Synonyms gene name
  • immunoglobulin kappa variable 2-29 (gene/pseudogene)
• GenBank acession
  • X63396
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28920
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151641

Gene info

• Identity
  HGNC:5800
• Gene
  IGKV2D-29
• Long gene name
  immunoglobulin kappa variable 2D-29
• GenBank acession
  • M31952
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28882
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151619

Gene info

• Identity
  HGNC:44982
• Gene
  ERVK-29
• Long gene name
  endogenous retrovirus group K member 29
• Synonyms gene name
  • endogenous retrovirus group K, member 29
• GenBank acession
  • JZ164912
• Locus
  19q13.12
• Discovery year
  2014-01-03
• Pubmed identfication
  • 23338945

Gene info

• Identity
  HGNC:49378
• Gene
  ERVW-29
• Long gene name
  endogenous retrovirus group W member 29
• Synonyms gene name
  • endogenous retrovirus group W, member 29
• GenBank acession
  • HG421066
• Locus
  18p11.21
• Discovery year
  2013-10-21

Gene info

• Identity
  HGNC:54433
• Gene
  RNVU1-29
• Long gene name
  RNA, variant U1 small nuclear 29
• Locus
  1q21.1
• Discovery year
  2019-07-26
• Classification
  • Variant U1 small nuclear RNAs

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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