Mouse Anti-Rat KCC2 Monoclonal IgG2a, Clone S1-12
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Catalog numberSMC-392D-BI
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PricePlease ask
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Size100 µg
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CloneS1-12
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ImmunogenFusion protein amino acids 932-1043 corresponding to rat KCC2
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Antibody s full descriptionMouse Anti-Rat KCC2 Monoclonal IgG2a Antibody, Clone: S1-12: Biotin
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Antibody s categoryMonoclonal Antibodies
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Antibody s other namePotassium Chloride Cotransporter Antibody, Potassium chloride transporter 5 Antibody, SLC12A5 Antibody, hKCC2 Antibody, S12A5 Antibody, Solute carrier family 12 member 5 Antibody, Electroneutral potassium-chloride cotransporter 2 Antibody, Furosemide-sensitive K-Cl cotransporter Antibody, K-Cl cotransporter 2 Antibody, rKCC2 Antibody, Neuronal K-Cl cotransporter Antibody, KIAA1176 Antibody, Electroneutral potassium chloride cotransporter 2 Antibody, Erythroid K Cl cotransporter 2 Antibody, Furosemide sensitive K Cl cotransporter Antibody, K-Cl cotransporter 2 Antibody, KCC 2 Antibody, Neuronal K Cl cotransporter Antibody, Solute carrier family 12 (potassium chloride transporter) member 5 Antibody, Solute carrier family 12 member 5 Antibody
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Raised inMouse
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Antibody s targetKCC2
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Primary research fieldsNeuroscience, Pumps/Transporters, Ion Pumps/Transporters
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:300), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~140kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-392 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextKCC2 is a member of the cation-chloride cotransporter gene family (1). It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain (2, 3). KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord (4). Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
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Bibliography1. Lee L.H., Walker J.A., Williams J.R., Goodier R.J., Payne J.A., Moss S.J. (2007) J Biol Chem. 282(41): 29777-29784. 2. Watanabe M., Wake H., Moorhouse A.J., Nabekura J. (2009) J Biol Chem. 284(41): 27980-27988. 3. Gulyas A.I., Sik A., Payne J.A., Kaila K., Freund T.F. (2001) Eur J Neurosci. 13(12): 2205-2217. 4. Vinay L., Jean-Xavier C. (2008) Brain Res Rev. 57(1): 103-110.
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Released date16-May-2012
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_599190
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Gene number171373
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Protein numberQ63633
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCC2 Monoclonal Antibody, Clone S1-12 (SMC-392). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody (SMC-392) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) KCC2 Antibody (D) Composite. Western Blot analysis of Rat brain membrane lysate showing detection of KCC2 protein using Mouse Anti-KCC2 Monoclonal Antibody, Clone S1-12 (SMC-392). Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody (SMC-392) at 1:1000. Mouse Anti-KCC2 Antibody [S1-12] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-392) Mouse Anti-KCC2 Antibody [S1-12] used in Western Blot (WB) on Rat brain membrane lysate (SMC-392)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolSLC12A5, PSMD1, PIRC55, PIRC54, PIRC53, PIRC57, PIRC56, SNORD116-12, IGHV3OR16-12
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Short nameMouse Anti- KCC2 Monoclonal IgG2a, Clone S1-12
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2a, IgG2a
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LabelBiotin
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat KCC2 monoclonal IgG2a, clonality S1-12
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Alternative techniquemurine, antibodies
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Clone nameS1-12
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Gene info
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Identity
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Gene
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Long gene namesolute carrier family 12 member 5
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Synonyms gene name
- solute carrier family 12 (potassium/chloride transporter), member 5
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-11-09
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Entrez gene record
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Classification
- Solute carriers
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameproteasome 26S subunit, non-ATPase 1
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Synonyms gene name
- proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-11-28
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Entrez gene record
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Pubmed identfication
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Classification
- Proteasome
- Armadillo like helical domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 55
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 54
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 53
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 57
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 56
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-12
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR16-12 (non-functional)
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Synonyms gene name
- immunoglobulin heavy variable 3/OR16-12
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data