Mouse Anti-Rat GluN2B/NR2B Monoclonal IgG2b, Clone S59-36

• Catalog number
  SMC-337D-A700
• Price
  Please ask
• Size
  100 µg

• Clone
  S59-36
• Immunogen
  Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B
• Antibody s full description
  Mouse Anti-Rat NR2B Monoclonal IgG2b Antibody, Clone: S59-36: ATTO 700
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  NMDAR2B Antibody, hNR3 Antibody, MGC142180 Antibody, glutamate receptor subunit epsilon-2 Antibody, NR3 Antibody
• Raised in
  Mouse
• Antibody s target
  NR2B
• Primary research fields
  Neuroscience, Ion Channels, Neurotransmitter Receptors, Glutamate Receptors, NMDA Receptors, Neurodegeneration
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF, IP, AM
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~166kDa. No cross-reactivity against NR2A.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  1 µg/ml of SMC-337 was sufficient for detection of NR2B in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  NR2B containing receptors have been implicated in synaptic plasticity, memory formation and pain modulation (1). Studies suggest that the NR2B subunit of glutamate receptors may be potential targets for relieving pain; NR2B may be a probable target for anti-nociceptive drugs, and may also be useful as analgesics (2).
• Bibliography
  1. Zhuo M. (2009) Mol Brain. 2(1): 4 2. Gurwitz D, and Weizman A. (2002) Drug Discovery Today, 7(7): 403-406.
• Released date
  31-Mar-2010
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_036706.1
• Gene number
  24410
• Protein number
  Q00960
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details | see here | read more
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 (SMC-337). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody (SMC-337) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive. Western Blot analysis of Human HEK-T lysates showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 (SMC-337). Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody (SMC-337) at 1:250. Immunohistochemistry analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 (SMC-337). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody (SMC-337) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining. Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 (SMC-337). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody (SMC-337) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-GluN2B/NR2B Antibody [S59-36] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-337) Mouse Anti-GluN2B/NR2B Antibody [S59-36] used in Western Blot (WB) on Human HEK-T lysates (SMC-337) Mouse Anti-GluN2B/NR2B Antibody [S59-36] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-337) Mouse Anti-GluN2B/NR2B Antibody [S59-36] used in Western Blot (WB) on Rat brain membrane lysate (SMC-337)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus

• Gene target
  GluN2B/NR2B   Monoclonal   IgG2b   S59-36  
• Gene symbol
  SNORD115-36, IGKV2D-36, IGHV3-36, IGKV2-36, IGLV1-36, SNORA35, RNU6-36P, PDLIM1, THAP4, ZNF608
• Short name
  Mouse Anti- GluN2B/NR2B Monoclonal IgG2b, Clone S59-36
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG2b, IgG2b
• Label
  ATTO 700
• Species
  Rat, Mouses, Rats
• Alternative name
  Mouse Antibody toRat GluN2B/NR2B monoclonal IgG2b, clonality S59-36
• Alternative technique
  murine, antibodies
• Clone name
  S59-36

Gene info

• Identity
  HGNC:33055
• Gene
  SNORD115-36
• Long gene name
  small nucleolar RNA, C/D box 115-36
• Synonyms
  • HBII-52-36
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033810
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003351.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5802
• Gene
  IGKV2D-36
• Long gene name
  immunoglobulin kappa variable 2D-36 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 2D-36
  • immunoglobulin kappa variable 2D-36 pseudogene
• GenBank acession
  • X71893
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28880
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151611

Gene info

• Identity
  HGNC:5599
• Gene
  IGHV3-36
• Long gene name
  immunoglobulin heavy variable 3-36 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 3-36
  • immunoglobulin heavy variable 3-36 pseudogene
• GenBank acession
  • M99667
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28431
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152068

Gene info

• Identity
  HGNC:5786
• Gene
  IGKV2-36
• Long gene name
  immunoglobulin kappa variable 2-36 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 2-36
  • immunoglobulin kappa variable 2-36 pseudogene
• GenBank acession
  • X71889
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28918
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151683

Gene info

• Identity
  HGNC:5876
• Gene
  IGLV1-36
• Long gene name
  immunoglobulin lambda variable 1-36
• GenBank acession
  • Z73653
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28826
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151074

Gene info

• Identity
  HGNC:32625
• Gene
  SNORA35
• Long gene name
  small nucleolar RNA, H/ACA box 35
• Synonyms
  • HBI-36
  • SNORA35A
• Locus
  Xq23
• Discovery year
  2006-04-04
• Entrez gene record
  677816
• Pubmed identfication
  • 15199136
  • 16381836
• RefSeq identity
  • NR_002993
• Classification
  • Small nucleolar RNAs, H/ACA box

Gene info

• Identity
  HGNC:34280
• Gene
  RNU6-36P
• Long gene name
  RNA, U6 small nuclear 36, pseudogene
• Synonyms gene
  • RNU6-36
• Synonyms gene name
  • RNA, U6 small nuclear 36
• Locus
  12q23.1
• Discovery year
  2008-06-10
• Entrez gene record
  100873757
• RefSeq identity
  • NR_046494

Gene info

• Identity
  HGNC:2067
• Gene
  PDLIM1
• Long gene name
  PDZ and LIM domain 1
• Synonyms gene
  • CLIM1
• Synonyms gene name
  • PDZ and LIM domain 1 (elfin)
• Synonyms
  • CLP-36
  • hCLIM1
  • CLP36
• Synonyms name
  • carboxyl terminal LIM domain protein 1
  • elfin
• GenBank acession
  • U90878
• Locus
  10q23.33
• Discovery year
  1998-01-21
• Entrez gene record
  9124
• Pubmed identfication
  • 10861853
• Classification
  • LIM domain containing
  • PDZ domain containing
• VEGA ID
  OTTHUMG00000018810

Gene info

• Identity
  HGNC:23187
• Gene
  THAP4
• Long gene name
  THAP domain containing 4
• Synonyms
  • CGI-36
  • Nb(III)
• Synonyms name
  • nitrobindin
• GenBank acession
  • AF258556
• Locus
  2q37.3
• Discovery year
  2003-10-08
• Entrez gene record
  51078
• Pubmed identfication
  • 12575992
  • 10810093
  • 30524950
• RefSeq identity
  • NM_015963
• Classification
  • THAP domain containing
• VEGA ID
  OTTHUMG00000133410

Gene info

• Identity
  HGNC:29238
• Gene
  ZNF608
• Long gene name
  zinc finger protein 608
• Synonyms
  • KIAA1281
  • DKFZp434M098
  • NY-REN-36
• GenBank acession
  • AB033107
• Locus
  5q23.2
• Discovery year
  2004-03-19
• Entrez gene record
  57507
• Pubmed identfication
  • 10574462
  • 10508479
• RefSeq identity
  • NM_020747
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000162999

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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